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| Status |
Public on Mar 31, 2010 |
| Title |
Global transcriptomic response of Leptospira interrogans serovar Copenhageni upon exposure to serum |
| Organism |
Leptospira interrogans |
| Experiment type |
Expression profiling by array
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| Summary |
L. interrogans, a causative agent of leptospirosis, can survive in the environment for lengthy periods of time in between infection of mammalian hosts. In order to identify genes involved in survival in the early spirochetemic phase of infection, we performed a transcriptional analysis of L. interrogans serovar Copenhageni upon exposure to serum in comparison with EMJH medium.
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| Overall design |
Analysis used RNA derived from serum- and EMJH-treated L. interrogans serovar Copenhageni as experimental and control samples, respectively. The samples were composed of 3 biological replicates with dye swap for each replicate, resulting in 6 arrays. Direct comparisons were made between arrays of experimental and control samples using raw data pulled from two different channels for data analysis.
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| Contributor(s) |
Patarakul K, Lo M, Adler B |
| Citation(s) |
20113507 |
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| Submission date |
Sep 02, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Kanitha Patarakul |
| E-mail(s) |
[email protected]
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| Phone |
662-256-4132
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| Fax |
662-252-5952
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| Organization name |
Faculty of Medicine, Chulalongkorn University
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| Department |
Microbiology
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| Street address |
1873 Rama IV Road
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| City |
Pathumwan |
| State/province |
Bangkok |
| ZIP/Postal code |
10330 |
| Country |
Thailand |
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| Platforms (1) |
| GPL9140 |
Chulalongkorn Univ Leptospira interrogans spotted 17K oligo array |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA119853 |
