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| Status |
Public on Jul 01, 2023 |
| Title |
Transcriptional effects of siRNA-mediated AhR knockdown on TLR7 activation of M-CSF-derived macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Analysis of the role of AhR on the transcriptional signature of TLR7-activated human M-CSF-dependent monocyte-derived macrophages. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures. Monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells). Monocytes from three independent donors (0.5 x 106 cells/ml, >95% CD14+ cells) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere with 5% CO2 and 21% O2 for 5 days in the presence of 1000 U/ml M-CSF, with cytokine addition every two days. Then, monocyte-derived macrophages were transfected with AhR-specific siRNA or control siRNA, kept in culture for 48 hours, and then stimulated (or not) with the TLR7 ligand CL264 (1 microgram/ml). After 4 hours, cells were lysed and RNA isolated for transcriptional analysis.
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| Overall design |
mRNA profiles of human M-CSF-derived macrophages activated with the TLR7 ligand CL264 after downregulation of AhR expession
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| Contributor(s) |
Simón Fuentes M, Domínguez Soto Á, Vega MA, Corbí AL |
| Citation(s) |
37040733 |
| |
| Submission date |
Jul 31, 2021 |
| Last update date |
Jul 06, 2023 |
| Contact name |
Angel L Corbí |
| E-mail(s) |
[email protected]
|
| Phone |
34 918373112
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| Organization name |
Consejo Superior de Investigaciones Científicas
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| Department |
Molecular Microbiology and Infection Biology
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| Lab |
Myeloid Cell Laboratory
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| Street address |
Ramiro de Maeztu 9
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| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
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| Platforms (1) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA751218 |
| SRA |
SRP330680 |
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