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| Status |
Public on Mar 16, 2022 |
| Title |
Transcriptional effects of LXR on the differentiation of monocyte-derived macrophages by tumor ascitic fluids or rheumatoid arthritis synovial fluids |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Analysis of the role of LXR on the transcriptional signature of monocyte-derived macrophages generated in the presence of tumor ascitic fluids or rheumatoid arthritis synovial fluids. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures. Monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells). Monocytes from independent donors (0.5 x 106 cells/ml, >95% CD14+ cells) were treated with DMSO (vehicle), 1 micromolar GW3965 or 1 micromolar GSK2033 for 1 hour, and then cultured in RPMI 1640 10% FBS supplemented with either 50% Tumor-derived Ascitic Fluid (TAF) or 20% Synovial Fluid from patients with active Rheumatoid Arthritis (RASF), at 37°C in a humidified atmosphere with 5% CO2 and 21% O2. After 72 hours, cells were lysed and RNA isolated for transcriptional analysis.
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| Overall design |
mRNA profiles of human monocyte-derived macrophages generated in the presence of tumor ascitic fluids or rheumatoid arthritis synovial fluids, and in the presence of LXR agonists or antagonists.
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| Contributor(s) |
González de la Aleja A, Castrillo A, Vega MA, Corbí AL |
| Citation(s) |
35280993, 36930354 |
| |
| Submission date |
Aug 02, 2021 |
| Last update date |
Jul 14, 2023 |
| Contact name |
Angel L Corbí |
| E-mail(s) |
[email protected]
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| Phone |
34 918373112
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| Organization name |
Consejo Superior de Investigaciones Científicas
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| Department |
Molecular Microbiology and Infection Biology
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| Lab |
Myeloid Cell Laboratory
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| Street address |
Ramiro de Maeztu 9
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| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
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| Platforms (1) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA751561 |
| SRA |
SRP330860 |
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