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| Status |
Public on Aug 08, 2021 |
| Title |
RNA seq of cardiac fibroblasts primary cell lines after stimulation with WNT3a or WNT5a with or without TGFB |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To recognize the influnence of WNT signaling on fibroblasts differentiation first we analyzed transformation of human cardiac fibroblasts caused by TGF-β signaling. Differential gene expression analysis demonstrated that cardiac fibroblasts 72h after treatment with TGF-β showed deregulated expression of 313 genes. We also observed that stimulation with WNT3a resulted in deregulation of 124 genes in TGF-β-treated fibroblasts and in contrast to profound effect of WNT3a on fibroblasts differentiation, treatment with WNT5a upregulated expression of only 2 and downregulated 21 genes in TGF-β-activated cells.
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| Overall design |
Examination of effect WNT3a and WNT5a on cardiac fibroblasts differentiation
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| |
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| Contributor(s) |
Działo E, Błyszczuk P |
| Citation(s) |
34576234 |
| |
| Submission date |
Aug 06, 2021 |
| Last update date |
Nov 07, 2021 |
| Contact name |
Przemysław Błyszczuk |
| E-mail(s) |
[email protected]
|
| Organization name |
Jagiellonian University
|
| Street address |
Wielicka 265
|
| City |
Kraków |
| ZIP/Postal code |
30-663 |
| Country |
Poland |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA752588 |
| SRA |
SRP331355 |
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