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| Status |
Public on Aug 31, 2021 |
| Title |
single cell RNA sequencing of CD8+CD44+GP33+ T cells from spleen and intraepithelial lymphocytes from small intestine at day 30 post LCMV Arm infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
During acute infections, CD8+ T cells form various memory subpopulations to provide long-lasting protection against reinfection. Central memory (TCM), Effector memory (TEM), and long-lived effector (LLE) cells are circulating memory populations with distinct plasticity, migration patterns, and effector functions. Tissue-resident memory (TRM) cells permanently reside in the frontline sites of pathogen entry and provide tissue-specific protection upon reinfection. Here, using scRNA-seq and bulk RNA-seq, we examined the different and shared transcriptomes and regulators of TRMs with other circulating memory populations. Furthermore, we identified heterogeneity within the TRM pool from small intestine and novel transcriptional regulators that may control the phenotypic and functional heterogeneity of TRM cells during acute infection. Our findings provide a resource for future studies to identify novel pathways for enhancing vaccination and immunotherapeutic approaches.
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| Overall design |
Two samples (CD8+CD44+GP33+ T cells from spleen and intraepithelial lymphocytes from small intestine) were prepared using 10x Genomics Chromium platform
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| Contributor(s) |
Chen Y, Cui W |
| Citation(s) |
34440912 |
| |
| Submission date |
Aug 10, 2021 |
| Last update date |
Sep 01, 2021 |
| Contact name |
Weiguo Cui |
| E-mail(s) |
[email protected]
|
| Phone |
312-503-0332
|
| Organization name |
Northwestern University
|
| Department |
Pathology
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| Lab |
Cui
|
| Street address |
303 E Chicago Ave
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60654 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE181785 |
Single-cell transcriptomics reveals core regulatory programs that determine the heterogeneity of circulating and tissue-resident memory CD8+ T cells |
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| Relations |
| BioProject |
PRJNA753473 |
| SRA |
SRP331891 |
