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| Status |
Public on Sep 23, 2021 |
| Title |
RNA-sequencing analysis of fluconazole-treated wild-type and upc2A null cells [Fluconazole] |
| Organism |
Nakaseomyces glabratus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Compare the fluconazole-induced transcriptome of isogenic wild-type and upc2A null cells.
Methods: Prepare total RNA from wild-type and upc2A null cells grown under unstressed conditions or challenged with 50 microgram/ml fluconazole for 6 hours. Use standard RNA-seq to evaluate and compare the transcriptomic changes caused by fluconazole in the presence or absence of UPC2A.
Results: These data established that over 100 genes were induced by fluconazole in a UPC2A-dependent manner. However, most fluconazole-induced genes (>500) did not require the presence of UPC2A. Fluconazole-induced genes included the expected genes involved in ergosterol biosynthesis but also large number of plasma membrane-localized transporters.
Conclusions: These data suggest that Upc2A provided transcriptional co-regulation between membrane transporters and the lipid environment in which these proteins function. A significant portion of the fluconazole-induced transcriptome does not require the presence of Upc2A, suggesting the presence of other transcriptional circuits responsive to this pharmacologic block of ergosterol biosynthesis.
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| Overall design |
Candida glabrata mRNA profiles of wild-type (WT) and upc2A null strains grown +/- fluconazole treatment.
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| Contributor(s) |
Li Y, Stamnes M, Moye-Rowley WS, Rogers PD |
| Citation(s) |
34591857 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI152494 |
Genetic analysis of pleiotropic drug resistance |
THE UNIVERSITY OF IOWA |
W Scott Moye-Rowley |
| R01 AI131620 |
Upc2A: A Central Regulator and "Achilles' Heel" of Fluconazole Resistance in Candida glabrata |
ST. JUDE CHILDREN'S RESEARCH HOSPITAL |
P. David Rogers |
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| |
| Submission date |
Aug 19, 2021 |
| Last update date |
Dec 27, 2021 |
| Contact name |
Scott Moye-Rowley |
| E-mail(s) |
[email protected]
|
| Phone |
3193357874
|
| Organization name |
University of Iowa
|
| Department |
Molecular Physiology & Biophysics
|
| Street address |
6-530 Bowen Science Bldg./Dept. of Mol Physio
|
| City |
Iowa City |
| State/province |
Iowa |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL22622 |
Illumina HiSeq 2000 ([Candida] glabrata) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE182516 |
Genomic level analysis of Upc2A-regulated transcription in Candida glabrata |
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| Relations |
| BioProject |
PRJNA756363 |
| SRA |
SRP333410 |
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