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| Status |
Public on Sep 23, 2021 |
| Title |
RNA-seq Analysis of Wild-Type and G898D UPC2A Transcriptomes [G898D UPC2A transcriptome] |
| Organism |
Nakaseomyces glabratus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Determine the number of genes that respond to the presence of a gain-of-function mutant form of the UPC2A transcription factor in Candida glabrata.
Methods: Prepare total RNA from wild-type and gain-of-function G898D UPC2A cells. Use standard RNA-seq to evaluate and compare the transcriptomic changes caused by this allele of UPC2A.
Results: Although a clear increase was seen in fluconazole resistance conferred by the G898D UPC2A allele, elevated transcription was only observed for 11 genes with 9 of these involved in ergosterol biosynthesis.
Conclusions: The presence of the G898D UPC2A allele led to elevated constitutive transcription for genes involved in ergosterol biosynthesis.
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| Overall design |
Candida glabrata mRNA profiles of wild-type (WT) and G898D UPC2A strains grown to mid-log phase.
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| Contributor(s) |
Li Y, Stamnes M, Moye-Rowley WS, Rogers PD |
| Citation(s) |
34591857 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI131620 |
Upc2A: A Central Regulator and "Achilles' Heel" of Fluconazole Resistance in Candida glabrata |
ST. JUDE CHILDREN'S RESEARCH HOSPITAL |
P. David Rogers |
| R01 AI152494 |
Genetic analysis of pleiotropic drug resistance |
THE UNIVERSITY OF IOWA |
W Scott Moye-Rowley |
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| |
| Submission date |
Aug 19, 2021 |
| Last update date |
Dec 27, 2021 |
| Contact name |
Scott Moye-Rowley |
| E-mail(s) |
[email protected]
|
| Phone |
3193357874
|
| Organization name |
University of Iowa
|
| Department |
Molecular Physiology & Biophysics
|
| Street address |
6-530 Bowen Science Bldg./Dept. of Mol Physio
|
| City |
Iowa City |
| State/province |
Iowa |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL22622 |
Illumina HiSeq 2000 ([Candida] glabrata) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE182516 |
Genomic level analysis of Upc2A-regulated transcription in Candida glabrata |
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| Relations |
| BioProject |
PRJNA756364 |
| SRA |
SRP333409 |
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