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| Status |
Public on Oct 28, 2009 |
| Title |
The effect of stpA deletion on S. Typhimurium gene expression during growth in rich medium |
| Platform organism |
Salmonella enterica |
| Sample organism |
Salmonella enterica subsp. enterica serovar Typhimurium str. SL1344 |
| Experiment type |
Expression profiling by array
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| Summary |
StpA is a paralogue of the nucleoid associated protein H-NS that is conserved in a range of enteric bacteria and had no known function in Salmonella enterica serovar Typhimurium. Here, we show that 5% of the Salmonella genome is regulated by StpA, which contrasts with the situation in Escherichia coli where deletion of stpA only had minor effects on gene expression. The StpA-dependent genes of S. Typhimurium are a specific subset of the H-NS regulon that are predominantly under the positive control of sigma38 (RpoS), CRP-cAMP and PhoP. The regulatory role of StpA varied at different growth phases; StpA only controlled sigma38 levels at mid-exponential phase when it prevented inappropriate activation of sigma38 during rapid bacterial growth. In contrast, StpA only activated the CRP-cAMP regulon during late exponential phase.
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| Overall design |
The effect of stpA deletion on S. Typhimurium gene expression during growth in LB was analysed at 4 different time points (early-log, mid-log, late-log, and stationary phase) where the gene expression profile of the stpA-deletion strain was compared to that of the parental strain. Between two and three biological replicates were performed for each strain and time point. For this study, we used Salmonella genomic DNA as the comparator which also acted as the control for spot quality.
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| Contributor(s) |
Lucchini S, McDermott P, Thompson A, Hinton JC |
| Citation(s) |
19843227 |
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| Submission date |
Oct 06, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Sacha Lucchini |
| E-mail(s) |
[email protected]
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| Organization name |
Institute of Food Research
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| Department |
Molecular Microbiology
|
| Street address |
Colney Lane
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| City |
Norwich |
| ZIP/Postal code |
NR4 7UA |
| Country |
United Kingdom |
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| Platforms (2) |
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| Samples (28)
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| This SubSeries is part of SuperSeries: |
| GSE18452 |
Role of stpA in Salmonella typhimurium |
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| Relations |
| BioProject |
PRJNA123333 |
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