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| Status |
Public on Oct 20, 2009 |
| Title |
Expression from dark grown Arabidopsis Wild type (Wt, Col-o) and ein3-1eil1-1 (AT3G20770, AT2G27050) mutant seedlings |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Ethylene is a gaseous hormone that plays important roles in plant growth, development and stress responses. EIN3 was identified as a plant-specific transcription factor and its protein level rapidly increases upon ethylene treatment. We found that activation of EIN3 and EIN3-like 1 (EIL1) is both necessary and sufficient for ethylene-induced enhancement of seedling greening, as well as repression of the accumulation of protochlorophyllide, a phototoxic intermediate of chlorophyll synthesis. Therefore, comparation of the Wt and ein3-1eil1-1 mutant etiolated seedlings' gene expression at genome wide will be helpfull for us to find the EIN3/EIL1 target genes in chlorophyll biosynthesis pathway. Here we used microarrays to detail the global gene expression under the regulation of EIN3/EIL1.Here we used microarrays to detail the global gene expression under the regulation of EIN3/EIL1.
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| Overall design |
4 days dark grown Wt and ein3-1eil1-1 (AT3G20770, AT2G27050) double mutant seedlings were collected for isolation RNA. Standard Affymetrix protocal and 25K Affymetrix chip (ATH1) were used for Microarry hybridization.
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| Contributor(s) |
Zhong S, An F, Guo H |
| Citation(s) |
19948955 |
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| Submission date |
Oct 19, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Shangwei Zhong |
| E-mail(s) |
[email protected]
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| Organization name |
Peking University, China
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| Department |
Life Science School
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| Lab |
New Life Science Building 428 room
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| Street address |
YiHeYuan West Street
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| City |
Peking |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (4)
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| GSM463014 |
ein3-1eil1-1, dark sample, biological rep |
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| Relations |
| BioProject |
PRJNA121401 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18631_RAW.tar |
11.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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