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| Status |
Public on Nov 15, 2009 |
| Title |
ChIP-chip of HA-tagged Lsr2 in Mycobacterium tuberculosis and Mycobacterium smegmatis |
| Platform organisms |
Mycobacterium tuberculosis H37Rv; Mycolicibacterium smegmatis MC2 155 |
| Sample organisms |
Mycolicibacterium smegmatis; Mycobacterium tuberculosis H37Rv |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Bacterial nucleoid-associated proteins play important roles in chromosome organization and global gene regulation. We find that Lsr2 of Mycobacterium tuberculosis is a novel nucleoid-associated protein that specifically binds AT-rich regions of the genome, including regions encoding major virulence factors, such as the ESX secretion systems, the lipid virulence factors PDIM/PGL, and the PE/PPE families of antigenic proteins. Comparison of genome-wide binding data with expression data indicates that Lsr2 binding results in transcriptional repression. Domain swamping experiments demonstrate that Lsr2 has an N-terminal dimerization domain and a C-terminal DNA binding domain. NMR analysis of the DNA binding domain of Lsr2 and its interaction with DNA reveals a novel structure and a unique mechanism that enables Lsr2 to discriminately target AT-rich sequences through interactions with the minor groove of DNA. Taken together, we provide evidence that mycobacteria have employed a structurally distinct molecule with an apparently different DNA recognition mechanism to achieve an equivalent function as the Enterobacteriaceae H-NS, coordinating global gene regulation and virulence in this group of medically important bacteria.
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| Overall design |
Comparison of Lsr2 chromatin-immunoprecipitated DNA sequences to total reference DNA
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| Contributor(s) |
Gordon BR, Li Y, Wang L, Sintsova A, van Bakel H, Tian S, Navarre WW, Xia B, Liu J |
| Citation(s) |
20133735 |
| |
| Submission date |
Oct 20, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Blair Gordon |
| E-mail(s) |
[email protected]
|
| Phone |
4169465068
|
| Organization name |
University of Toronto
|
| Department |
Molecular Genetics
|
| Lab |
Liu Lab
|
| Street address |
1 King's College Circle
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
m5s1a8 |
| Country |
Canada |
| |
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| Platforms (2) |
| GPL9467 |
Mycobacterium tuberculosis H37Rv 241K Whole Genome Tilling Array |
| GPL9468 |
Mycobacterium smegmatis MC2 155 241K Whole Genome Tilling Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA121589 |
