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| Status |
Public on Nov 02, 2021 |
| Title |
Single cell RNA sequencing analysis of midbrain organoids with SNCA gene triplication modeling key features of synuclienopathy |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
SNCA, the first gene associated with Parkinson’s disease, encodes the α-synuclein protein, the predominant component within pathological inclusions termed Lewy bodies. We use 3D midbrain organoids, differentiated from human induced pluripotent stem cells derived from patients carrying a triplication of the SNCA gene and from CRISPR/Cas9 corrected isogenic control iPSCs. These human midbrain organoids recapitulate key features of α-synuclein pathology observed in the brains of patients with synucleinopathies. We used single cell RNA sequencing to characterize the cell types within these organoids. We find an equal proportion of neuronal and glial cells. The neuronal populations consist of dopmainergic, excitatory and inhibitory neurons in early and late stages of maturation as well as neural precursor cells. The glial populations consist of dividing radial glia, radial glia, astrocytes and early oligodendorcytes.
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| Overall design |
Single cell RNA sequencing transcriptomes of midbrain organoids 165 days in shaking cultures. Two sequencing libraries one for each genotype, each sample is 3 midbrain organoids pooled and dissociated.
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| Contributor(s) |
Thomas RA, Mohamed N, Durcan TM |
| Citation(s) |
34632384 |
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| Submission date |
Oct 28, 2021 |
| Last update date |
Nov 03, 2021 |
| Contact name |
Rhalena Amber Thomas |
| E-mail(s) |
[email protected]
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| Phone |
5147137513
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| Organization name |
McGill University
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| Department |
Neurology
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| Lab |
Edward Fon
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| Street address |
3801 rue University, Montreal Neurological Institute, NW B150
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| City |
Montreal |
| State/province |
QC |
| ZIP/Postal code |
H3A 2B4 |
| Country |
Canada |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA776008 |
| SRA |
SRP343656 |
