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| Status |
Public on Oct 23, 2009 |
| Title |
Expression data from murine embryonic stem (ES) cells exposed to oxidative stress |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Elevated reactive oxidative species (ROS) may be crucial for cellular transformation and tumor progression. Defining the response of stem cells to ROS is critical to understanding the unique sensitivity of stem cells to ROS-mediated transformation and tumor initiation.
We examined both the immediate transcriptional and phospho-proteomic responses of murine ES cells induced by a single brief dose of hydrogen peroxide (H2O2) to create a more complete understanding of cell response to ROS.
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| Overall design |
Murine ES cells were briefly exposed to low and high dose of H2O2 then allowed to recover for 1 hour in maintenance medium before RNA extraction and MG_U74Av2 microarray hybridization.
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| Contributor(s) |
Richardson C, Mouzannar R |
| Citation(s) |
21743783 |
| |
| Submission date |
Oct 23, 2009 |
| Last update date |
Aug 19, 2019 |
| Contact name |
Christine Richardson |
| Organization name |
Columbia University
|
| Department |
Institute for Cancer Genetics
|
| Lab |
Richardson
|
| Street address |
St Nicholas Av
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL81 |
[MG_U74Av2] Affymetrix Murine Genome U74A Version 2 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA121503 |
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