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| Status |
Public on Dec 10, 2021 |
| Title |
Age-related changes of gene expression profiles in Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
An individual’s gene expression profile changes throughout life. This change in gene expression is shaped by differences in physiological needs and functions between the younger and older organism. Here we report a transcriptomic analysis of Drosophila melanogaster using microarrays. We compared the expression profiles of two-day-old female adult flies with those of 45-day-old flies. We identified 1184 genes with pronounced expression level differences between young and old age groups. Most genes involved in muscle development/maintenance that display different levels of expression with age were downregulated in older flies. Many of these genes contributed to sarcomere formation and function. Several of these genes were functionally related to direct and indirect flight muscles; some of them were exclusively expressed in these muscles. Conversely, several genes involved in apoptosis processes were upregulated in aging flies. Close to half of these genes, such as different caspases, promote cell death, whereas the other half is associated with the inhibition of apoptosis. Several genes that inhibit apoptosis contribute to cell-type maintenance for different cell groups, including neurons, cardiac cells, and salivary gland cells. In addition, several genes involved in resistance to toxic chemicals were upregulated in aging flies, which is consistent with a global upregulation of the defense response system in aging flies. Finally, 232 of the differentially expressed genes had no known functions.
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| Overall design |
In total 6 samples: 3 biological replicates of 3 days-old flies and 3 biological replicates of 45 days-old flies
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| Contributor(s) |
Guillaume B, Niraj L, Andrew KV, Alexei T |
| Citation(s) |
34946931 |
| |
| Submission date |
Nov 03, 2021 |
| Last update date |
Mar 11, 2022 |
| Contact name |
Alexei Tulin |
| E-mail(s) |
[email protected]
|
| Organization name |
University of North Dakota
|
| Department |
Epigenetics Department
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| Lab |
Alexei Tulin Lab
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| Street address |
501 N Columbia Rd Stop 9061
|
| City |
Grand Forks |
| State/province |
North Dakota |
| ZIP/Postal code |
58203-2898 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA777691 |
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