|
| Status |
Public on Nov 06, 2021 |
| Title |
TNF plays a crucial role in inflammation by signaling via T cell TNFR2 [bulkRNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
TNF regulates the inflammatory cytokine secretion from CD4+ T cells in vitro.
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| |
|
| Overall design |
In vitro experiment (Bulk seq): Naïve CD4+ T cells from WT or Tnfr2-/- mice were isolated from spleen and stimulated with anti-CD3/CD28 for 3 days in the presence of the different combinations of cytokines (WT: TNF+TGF-β, Tnfr2-/-: TNF+TGF-β; WT: IL-6+TGF-β) followed by bulk RNA sequence. Each experimental condition was done triplicates and analyzed.
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| |
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| Contributor(s) |
Alam MS, Wong N, Fan Y, Ashwell JD |
| Citation(s) |
34873037 |
| |
| Submission date |
Nov 04, 2021 |
| Last update date |
Feb 07, 2022 |
| Contact name |
Jonathan D Ashwell |
| E-mail(s) |
[email protected]
|
| Phone |
240 760-6865
|
| Organization name |
NIH
|
| Department |
Laboratory of Immune Cell Development
|
| Street address |
Room 3002, Bldg. 37, 9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE188162 |
TNF plays a crucial role in inflammation by signaling via T cell TNFR2 |
|
| Relations |
| BioProject |
PRJNA777869 |
| SRA |
SRP344554 |
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