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| Status |
Public on Dec 06, 2021 |
| Title |
An instructive role for Interleukin-7 receptor α in the development of human B-cell precursor leukemia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Bulk RNAseq for transplanted transduced (CRLF2-IL7RAins or BB control)CB cells
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| Overall design |
Total RNA was purified from 5000-20000 transduced (CRLF2+ or GFP+) CD45+CD3- cells that were sorted from spleens of transplanted mice. cDNA libraries were prepared using SMARTer v3 kit (Clontech Laboratories, Inc. A Takara Bio Company, Mountain View, CA, USA) followed by the Nextera-XT indexing protocols (Illumina, CA, USA). Genome-wide expression profiles were obtained by sequencing of the samples on Illumina NextSeq 500 using NextSeq 500/550 High Output v2 kit.
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| |
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| Contributor(s) |
Geron I, Brown J, Izraeli S, Enver T |
| Citation(s) |
35115489 |
| |
| Submission date |
Dec 02, 2021 |
| Last update date |
Feb 18, 2022 |
| Contact name |
Dan Ben-Avraham |
| E-mail(s) |
[email protected]
|
| Organization name |
Weizmann Institute of Science
|
| Department |
Grand Israel National Center for Personalized Medicine
|
| Lab |
Mantoux Institute for Bioinformnatics
|
| Street address |
Hertzel 234
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| City |
Rehovot |
| ZIP/Postal code |
7610001 |
| Country |
Israel |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA785656 |
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