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| Status |
Public on Nov 23, 2010 |
| Title |
Expression Analysis of Geobacter sulfurreducens Current-Consuming Biofilm versus Current-Producing Biofilm |
| Organism |
Geobacter sulfurreducens |
| Experiment type |
Expression profiling by array
|
| Summary |
There is a wide diversity of potential applications for direct electron transfer from electrodes to microorganisms, which might be better optimized if the mechanisms for this novel electrode-biofilm interaction were better understood. Geobacter sulfurreducens is one of the few microorganisms available in pure culture that is known to be capable of directly accepting electrons from a negatively poised electrode. A microarray comparison of cells accepting electrons from the electrode versus cells donating electrons to the electrode reveals that the genes previously observed to be upregulated in current-producing biofilms are not highly expressed in current-consuming biofilms.
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| Overall design |
Current-consuming biofilms were grown under anoxic conditions in freshwater medium in a potentiostat-poised (-500 mV vs. Ag/AgCl), dual-chambered electrode system where the electrode served as the electron donor and fumarate as the electron acceptor. Current-producing biofilms were grown under identical condition, except the electrode served as the electron acceptor (+300 mV vs. Ag/AgCl) and acetate as the electron donor. Triplicate electrode systems of either current-consuming biofilms or current-producing biofilms were harvested for total RNA.
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| Contributor(s) |
Strycharz SM, Nevin KP, Perpetua LA, Liu A, Lovley DR |
| Citation(s) |
20696622 |
| |
| Submission date |
Nov 23, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Sarah Strycharz |
| E-mail(s) |
[email protected]
|
| Phone |
202-767-3822
|
| Fax |
202-334-2759
|
| Organization name |
Naval Research Laboratory
|
| Department |
CBMSE
|
| Lab |
Tender
|
| Street address |
4555 Overlook Ave.
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20375 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL4897 |
Geobacter sulfurreducens sense-detecting 12K oligo array |
|
| Samples (6)
|
| GSM474702 |
PCA_donorvsacceptor_potentiostat_BR1A |
| GSM474703 |
PCA_donorvsacceptor_potentiostat_BR1B |
| GSM474704 |
PCA_donorvsacceptor_potentiostat_BR2A |
| GSM474705 |
PCA_donorvsacceptor_potentiostat_BR2B |
| GSM474706 |
PCA_donorvsacceptor_potentiostat_BR3A |
| GSM474707 |
PCA_donorvsacceptor_potentiostat_BR3B |
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| This SubSeries is part of SuperSeries: |
| GSE19158 |
Geobacter sulfurreducens biofilm transcriptomics |
|
| Relations |
| BioProject |
PRJNA123753 |
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