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Series GSE19357 Query DataSets for GSE19357
Status Public on Dec 15, 2009
Title Gene expression analysis of bone tumors
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Subungual exostosis is a benign bone- and cartilage-forming tumor known to harbour a pathognomonic t(X;6)(q22;q13-14). Using global gene expression analysis and quantitative real-time PCR we could show that this translocation results in increased expression of the IRS4 gene, presumably due to disruption and/or exchange of regulatory sequences with the translocation partner, the COL12A1 gene. A corresponding deregulation at the protein level could be demonstrated in primary cell cultures using a combination of fluorescence in situ hybridization and immunostaining. As the t(X;6) usually is the sole cytogenetic aberration in subungual exostosis, the deregulated expression of IRS4 is likely to be pathogenetically essential.
Gene expression analysis of bone tumors.
 
Overall design Total RNA was extracted from five frozen bone tumor biopsies (two osteochondromas, two cases of BPOP, one subungual exostosis), cDNA was generated, fragmented and end-labelled. The fragmented and biotinylated cDNA was hybridized to the GeneChipR Human Gene 1.0 ST Arrays (Affymetrix).
 
Contributor(s) Mertens F
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Submission date Dec 07, 2009
Last update date Jul 26, 2018
Contact name Fredrik Mertens
E-mail(s) [email protected]
Organization name Lund University
Department Dept of Clinical Genetics
Lab Lund University Hospital
Street address Lund University Hospital
City Lund
ZIP/Postal code 22185
Country Sweden
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (5)
GSM480608 Subungual exostosis 1
GSM480609 Osteochondroma 7
GSM480610 Osteochondroma 8
Relations
BioProject PRJNA120391

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19357_RAW.tar 20.2 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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