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Series GSE193675 Query DataSets for GSE193675
Status Public on Jun 27, 2022
Title Gene expression in Day-8 equine embryos from young nulliparous mares
Organism Equus caballus
Experiment type Expression profiling by high throughput sequencing
Summary The aim of the global study was to compare the effects of maternal age and parity on gene expression of equine embryos. For that, 17 embryos were collected at 8 days post ovulation (expanded blastocyst stage) from 3 groups of young or old nulliparous or multiparous mares: 6 from young nulliparous mares (5 or 6 year-old, never foaled before), 5 from young multiparous mares (6 year-old and foaled at least once) and 6 from old multiparous (10-16 year-old and foaled at least once). Embryos collected were cut in 2 parts: one hemi-embryo containing pure trophoblast and the other one containing trophoblast + inner cell mass. ARN extractions were performed for all samples using PicoPure RNA isolation kit (Applied Biosystem). Five nanograms of both parts of each embryo were sequenced in paired-end with a length of 30-50bp separately using Illumina NextSeq 500 High. Data were trimmed using Cutadapt. Sequences with less than 10bp were removed. Data for young and old multiparous mares had been already published in [accession: GSE162893; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE162893]. Only data for embryos collected from young nulliparous mares are deposited here
 
Overall design 6 embryos cut in ICM and TE parts analyzed according to maternal parity (Nulliparous or multiparous mares)
 
Contributor(s) Derisoud E, Jouneau L, Dubois C, Archilla C, Jaszczyszyn Y, Legendre R, Daniel N, Peynot N, Dahirel M, Wimel L, Duranthon V, Chavatte-Palmer P
Citation(s) 35705916
Submission date Jan 13, 2022
Last update date Jun 27, 2022
Contact name Emilie Derisoud
E-mail(s) [email protected], [email protected]
Organization name INRAE
Street address 26 Rue des Prés,
City PALAISEAU
State/province Essonne
ZIP/Postal code 91120
Country France
 
Platforms (1)
GPL21401 Illumina NextSeq 500 (Equus caballus)
Samples (12)
GSM5816040 YN, Embryo 1, ICM
GSM5816041 YN, Embryo 1, TE
GSM5816042 YN, Embryo 2, ICM
This SubSeries is part of SuperSeries:
GSE193676 EPAJ: Effect of mares' parity and age
Relations
BioProject PRJNA797169

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE193675_counts_CH_YN.txt.gz 2.8 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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