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| Status |
Public on Apr 19, 2022 |
| Title |
CUT&Tag for H2A.Z using mouse heart tissues from control and Znhit1-deficient mice |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Znhit1 is an important subunit of the SRCAP complex, which regulates gene transcription by replacing histone H2A with a variant H2A.Z at gene promoter regions. Deletion of Znhit1 has also been reported to impact the incorporation of H2A.Z in genome. To explore the function of Znhit1 in heart tissues, we performed CUT&Tag assays for H2A.Z to examine the distribution changes of H2A.Z on the genome.
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| Overall design |
CUT&Tag assays for H2A.Z using mouse heart tissues from control (Znhit1 flox/flox) and Znhit1 cKO (Znhit1 flox/flox; Myh6-Cre) mice at postnatal day 17 (P17) and postnatal day 25 (P25).
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| Contributor(s) |
Shi Y, Yang Z |
| Citation(s) |
35167494 |
| |
| Submission date |
Jan 21, 2022 |
| Last update date |
Apr 20, 2022 |
| Contact name |
Yingchao Shi |
| E-mail(s) |
[email protected]
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| Phone |
15261891962
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| Organization name |
Nanjing University
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| Street address |
22 Hankou Road
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| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210093 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (9)
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| GSM5829868 |
Control heart tissues for H2A.Z CUT&Tag at P25_rep1 |
| GSM5829869 |
Control heart tissues for H2A.Z CUT&Tag at P25_rep2 |
| GSM5829870 |
Znhit1-cKO heart tissues for H2A.Z CUT&Tag at P25 |
| GSM5829871 |
Control heart tissues for H2A.Z CUT&Tag at P17 |
| GSM5829872 |
Znhit1-cKO heart tissues for H2A.Z CUT&Tag at P17 |
| GSM5829873 |
Control heart tissues for H3K27me3 CUT&Tag at P17 |
| GSM5829874 |
Znhit1-cKO heart tissues for H3K27me3 CUT&Tag at P17 |
| GSM5829875 |
Control heart tissues for H3K4me3 CUT&Tag at P17 |
| GSM5829876 |
Znhit1-cKO heart tissues for H3K4me3 CUT&Tag at P17 |
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| This SubSeries is part of SuperSeries: |
| GSE194164 |
Znhit1-deficiency caused sarcoplasmic reticulum originated vacuolar cardiomyopathy |
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| Relations |
| BioProject |
PRJNA799339 |
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