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| Status |
Public on Feb 09, 2022 |
| Title |
mRNA transcriptome sequencing of wild type (WT) and intestinal epithelial cell specific Axin1 Knockout mice (Axin1KOΔIEC) that have udergone DSS-induced colitis and AOM/DSS-induced colorectal tumorigenesis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose : The goals of this study are to compare NGS-derived transcriptome profiling (RNA-seq) of colon samples of intestinal epithelial cell specific Axin1 Knockout mice and WT controls that were submitted to DSS-induced colitis and AOM/DSS-induced colorectal carcinogenesis.
Methods : DSS-induced colitis was performed on Axin1flfl (WT) and Vil CreERT2;Axin1fl/fl (Axin1KOΔIEC) mice by giving 3% DSS dissolved in drinking water for 7 days and subsequently placed on regular water for recovery before sacrifice at Day 7 and D13.
Methods : AOM/DSS-induced colorectal tumorigenesis was performed on Axin1flfl (WT) and Vil CreERT2;Axin1fl/fl (Axin1KOΔIEC) mice that were sacrificed at day 100 post-AOM injection to collect colorectal tumors.
Methods : Colonic mRNA profiles of WT and Axin1KOΔIEC mice were generated by deep sequencing using Illumina NextSeq 500 instrument (150base-lengths read V2 chemistry in a paired-end mode)
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| Overall design |
Colonic mRNA profiles of wild type (WT) and intestinal epithelial cell specific Axin1 Knockout mice that have udergone DSS-induced colitis. Three biological replicates for each group.Colorectal tumor mRNA profiles of wild type (WT) and intestinal epithelial cell specific Axin1 Knockout mice that have undergone AOM/DSS-induced colorectal tumorigenesis. Three biological replicates for each group
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| Contributor(s) |
Sansom R, Romagnolo B, Perret C |
| Citation(s) |
36356835 |
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| Submission date |
Feb 06, 2022 |
| Last update date |
Feb 07, 2023 |
| Contact name |
Christine Perret |
| E-mail(s) |
[email protected]
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| Phone |
33144412564
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| Organization name |
Institut Cochin
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| Department |
DRC
|
| Street address |
24, rue du faubourg Saint-Jacques
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| City |
PARIS |
| ZIP/Postal code |
75014 |
| Country |
France |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA803912 |
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