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| Status |
Public on Sep 01, 2010 |
| Title |
Vascular gene expression in mice overexpressing human endothelin-1 targeted to the endothelium |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Endothelin-1 (ET-1), an endothelium-derived vasoconstrictor peptide, plays a role in the pathophysiology of cardiovascular disease. Transgenic mice that overexpress human preproET-1 selectively in the endothelium (eET-1) exhibit endothelial dysfunction, hypertrophic remodeling and vascular inflammation of resistance-size arteries in the absence of blood pressure elevation. To understand the mechanisms whereby ET-1 induces vascular damage, vascular gene expression using DNA microarrays was employed. RNA from mesenteric arteries of female and male young (6-7 weeks) and mature (6-8 months) eET-1 and wild type (WT) mice was isolated and changes in gene expression were determined by genome-wide expression profiling using Illumina microarray. This study revealed a set of genes potentially regulated by ET-1, which might be implicated in ET-1 induced-vascular damage.
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| Overall design |
Samples 1-8: Total RNA obtained from the entire mesenteric arterial tree of young (6-7 weeks) female preproendothelin-1 overexpressing mice compared to age and sex matched wild type littermate controls.
Samples 9-16: Total RNA obtained from the entire mesenteric arterial tree of mature (6-8 months) female preproendothelin-1 overexpressing mice compared to age and sex matched wild type littermate controls.
Samples 17-24: Total RNA obtained from the entire mesenteric arterial tree of young (6-7 weeks) male preproendothelin-1 overexpressing mice compared to age and sex matched wild type littermate controls.
Samples 25-32: Total RNA obtained from the entire mesenteric arterial tree of mature (6-8 months) male preproendothelin-1 overexpressing mice compared to age and sex matched wild type littermate controls.
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| Contributor(s) |
Simeone SM, Paradis P, Schiffrin EL |
| Citation(s) |
21045115 |
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| Submission date |
Dec 23, 2009 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Stefania Simeone |
| E-mail(s) |
[email protected]
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| Phone |
514-340-8222
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| Organization name |
Lady Davis Institute for Medical Research
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| Department |
Hypertension and Vascular Research Unit
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| Lab |
Dr. Ernesto L Schiffrin
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| Street address |
3755 Côte Ste-Catherine Road
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| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3T 1E2 |
| Country |
Canada |
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| Platforms (2) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
| GPL8389 |
Illumina Mouse-8 Expression BeadChip |
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| Samples (32)
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| GSM489305 |
mesenteric arterial tree, eET-1 mouse, replicate 1, female young |
| GSM489306 |
mesenteric arterial tree, eET-1 mouse, replicate 2, female young |
| GSM489307 |
mesenteric arterial tree, eET-1 mouse, replicate 3, female young |
| GSM489308 |
mesenteric arterial tree, eET-1 mouse, replicate 4, female young |
| GSM489309 |
mesenteric arterial tree, WT mouse, replicate 1, female young |
| GSM489310 |
mesenteric arterial tree, WT mouse, replicate 2, female young |
| GSM489311 |
mesenteric arterial tree, WT mouse, replicate 3, female young |
| GSM489312 |
mesenteric arterial tree, WT mouse, replicate 4, female young |
| GSM489313 |
mesenteric arterial tree, eET-1 mouse, replicate 1, female mature |
| GSM489314 |
mesenteric arterial tree, eET-1 mouse, replicate 2, female mature |
| GSM489315 |
mesenteric arterial tree, eET-1 mouse, replicate 3, female mature |
| GSM489316 |
mesenteric arterial tree, eET-1 mouse, replicate 4, female mature |
| GSM489317 |
mesenteric arterial tree, WT mouse, replicate 1, female mature |
| GSM489318 |
mesenteric arterial tree, WT mouse, replicate 2, female mature |
| GSM489319 |
mesenteric arterial tree, WT mouse, replicate 3, female mature |
| GSM489320 |
mesenteric arterial tree, WT mouse, replicate 4, female mature |
| GSM489321 |
mesenteric arterial tree, eET-1 mouse, replicate 1, male young |
| GSM489322 |
mesenteric arterial tree, eET-1 mouse, replicate 2, male young |
| GSM489323 |
mesenteric arterial tree, eET-1 mouse, replicate 3, male young |
| GSM489324 |
mesenteric arterial tree, eET-1 mouse, replicate 4, male young |
| GSM489325 |
mesenteric arterial tree, WT mouse, replicate 1, male young |
| GSM489326 |
mesenteric arterial tree, WT mouse, replicate 2, male young |
| GSM489327 |
mesenteric arterial tree, WT mouse, replicate 3, male young |
| GSM489328 |
mesenteric arterial tree, WT mouse, replicate 4, male young |
| GSM489329 |
mesenteric arterial tree, eET-1 mouse, replicate 1, male mature |
| GSM489330 |
mesenteric arterial tree, eET-1 mouse, replicate 2, male mature |
| GSM489331 |
mesenteric arterial tree, eET-1 mouse, replicate 3, male mature |
| GSM489332 |
mesenteric arterial tree, eET-1 mouse, replicate 4, male mature |
| GSM489333 |
mesenteric arterial tree, WT mouse, replicate 1, male mature |
| GSM489334 |
mesenteric arterial tree, WT mouse, replicate 2, male mature |
| GSM489335 |
mesenteric arterial tree, WT mouse, replicate 3, male mature |
| GSM489336 |
mesenteric arterial tree, WT mouse, replicate 4, male mature |
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| Relations |
| BioProject |
PRJNA122579 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19624_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR |
| GSE19624_SampleProbeProfile_Female_Mature.txt.gz |
5.7 Mb |
(ftp)(http) |
TXT |
| GSE19624_SampleProbeProfile_Female_Young.txt.gz |
2.5 Mb |
(ftp)(http) |
TXT |
| GSE19624_SampleProbeProfile_Male_Mature.txt.gz |
6.5 Mb |
(ftp)(http) |
TXT |
| GSE19624_SampleProbeProfile_Male_Young.txt.gz |
5.7 Mb |
(ftp)(http) |
TXT |
| GSE19624_female_mature_RAW.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| GSE19624_female_young_RAW.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| GSE19624_male_mature_RAW.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| GSE19624_male_young_RAW.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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