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| Status |
Public on Feb 12, 2022 |
| Title |
LncRNAs expression profiling in small extracellular vesicles derived from brown adipose tissue |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
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| Summary |
Brown adipose tissue (BAT) is considered as a main site of adaptive thermogenesis and the thermogenic activities of brown and beige adipocytes are also linked to generating heat and counteracting obesity. Recent studies revealed that BAT could secrete certain batokines-like factors especially small extracellular vesicles (sEV), which contributed to the systemic consequences of BAT activities. As a newly emerging class of mediators, some long non-coding RNAs (lncRNAs) have exhibited metabolic regulatory effects in adipocyte development. However, besides the well-studied lncRNAs, the lncRNAs carried by sEV derived from brown adipose tissue (sEV-BAT) have not been characterized yet. In this study, we conducted a lncRNA microarray assay on sEV-WAT and sEV-BAT. A total of 563 types of known lncRNAs were identified to be differentially expressed, among which 232 lncRNAs were upregulated and 331 lncRNAs were downregulated in sEV-BAT. Three novel candidates (AK029592, humanlincRNA1030 and ENSMUST00000152284) were selected for further validation. LncRNA–mRNA network analysis revealed candidate lncRNAs were largely embedded in cellular metabolic pathways. During adipogenic and thermogenic phenotype differentiation in ASCs and 3T3-L1 cells, only the expressions of AK029592 were upregulated. The three lncRNAs were all relatively enriched in brown adipose tissues and brown adipocytes. In different adipocytes, sEV and adipose tissue, the expression of AK029592 and ENSMUST00000152284 were significantly decreased in obese mice compared to lean controls, while obesity could not alter the expression of humanlincRNA1030.
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| Overall design |
LncRNAs expression were measured in sEV-BAT and sEV-WAT from C57BL/6 male mice with three replicates using Arraystar Mouse LncRNA Arrays V4.0
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| Contributor(s) |
Hong P, Wu Y, Yu M, Tian W |
| Citation(s) |
36117155, 39115701 |
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| Submission date |
Feb 10, 2022 |
| Last update date |
Nov 05, 2024 |
| Contact name |
Pengyu Hong |
| E-mail(s) |
[email protected]
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| Organization name |
West China School of Stomatology, Sichuan University
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| Street address |
No.14, Section 3, Renmin South Road
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| City |
Chengdu |
| ZIP/Postal code |
610001 |
| Country |
China |
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| Platforms (1) |
| GPL26962 |
Agilent-085983 Arraystar Mouse lncRNA V4 microarray |
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| Samples (6)
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| GSM5884690 |
small extracellular vesicles derived from brown adipose tissue_rep1 |
| GSM5884691 |
small extracellular vesicles derived from brown adipose tissue_rep2 |
| GSM5884692 |
small extracellular vesicles derived from brown adipose tissue_rep3 |
| GSM5884693 |
small extracellular vesicles derived from white adipose tissue_rep1 |
| GSM5884694 |
small extracellular vesicles derived from white adipose tissue_rep2 |
| GSM5884695 |
small extracellular vesicles derived from white adipose tissue_rep3 |
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| Relations |
| BioProject |
PRJNA805100 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE196468_RAW.tar |
18.3 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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