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| Status |
Public on May 12, 2022 |
| Title |
Nucleosome sliding by the Chd1 chromatin remodeler relies on the integrity of the DNA duplex |
| Organism |
synthetic construct |
| Experiment type |
Other
|
| Summary |
The experiments are done with three libraries by introducing base-pair perturbations on Widom 601: (1) poly(dA:dT) tract library (2) mismatch library (3) insertion library. And additional two more libraries based on native yeast genomic sequences: (4) Park97 mismatch library (5) Park97 insertion library. And we measured the nucleosome positioining in the base-pair resolution for before and after sliding by Chd1 chromatin remodeler.
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| Overall design |
The experiments are done with three libraries by introducing base-pair perturbations on Widom 601: (1) poly(dA:dT) tract library (2) mismatch library (3) insertion library. And additional two more libraries based on native yeast genomic sequences: (4) Park97 mismatch library (5) Park97 insertion library. And we measured the nucleosome positioining in the base-pair resolution for before and after sliding by Chd1 chromatin remodeler.
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| |
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| Contributor(s) |
Park S, Brandani GB, Ha T, Bowman G |
| Citation(s) |
37738162 |
| |
| Submission date |
Mar 11, 2022 |
| Last update date |
Nov 13, 2023 |
| Contact name |
Taekjip Ha |
| Organization name |
Johns Hopkins University
|
| Street address |
3400 N. Charles Street
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21218 |
| Country |
USA |
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| Platforms (3) |
| GPL17769 |
Illumina MiSeq (synthetic construct) |
| GPL19604 |
Illumina HiSeq 2500 (synthetic construct) |
| GPL26526 |
Illumina NovaSeq 6000 (synthetic construct) |
|
| Samples (30)
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| Relations |
| BioProject |
PRJNA815305 |
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