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| Status |
Public on Mar 17, 2022 |
| Title |
RNA-sequencing analysis of the KYSE150 cells overexpressing caspase-8 mutants (M4, M6, M10, M13) or wild-type caspase-8 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are using RNA sequencing analysis to test the effects of overexpressing caspase-8 mutants or wild-type caspase-8 on the mRNA of KYSE150 cells. Methods: KYSE150 cells were transfected with caspase-8 mutants or wild-type caspase-8 for 48 hours in 1640 medium plus 10% serum. Two independent replicates were plated, transfected in parallel for each caspase-8 mutants or wild-type caspase-8. Results: Log-fold changes of mRNAs between caspase-8 mutants and wild-type caspase-8 group were selected with a significance threshod of p<0.05. There are 3974 overlapping differentially expressed genes in the caspase-8 mutant cells compared to the wild-type caspase-8 cells. Conclusions: Our study determined the mRNA changes of caspase-8 mutant KYSE150 cells compared to the wild-type caspase-8 cells.
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| Overall design |
KYSE150 cells mRNA profiles of WT (transfected with wild-type caspase-8), M4 (transfected with caspase-8 M4 mutant), M6 (transfected with caspase-8 M6 mutant), M10 (transfected with caspase-8 M10 mutant), M13 (transfected with caspase-8 M13 mutant) were generated by RNA sequencing.
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| Contributor(s) |
Lei L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Mar 14, 2022 |
| Last update date |
Mar 18, 2022 |
| Contact name |
li lei |
| E-mail(s) |
[email protected]
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| Organization name |
Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College
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| Street address |
17 Panjiayuan Nanli
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| City |
beijing |
| ZIP/Postal code |
100021 |
| Country |
China |
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| Platforms (1) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA816048 |
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