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| Status |
Public on Jun 21, 2022 |
| Title |
Gene-regulatory potential of 25-hydroxyvitamin D3 and D2 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, we compared the modulation of the transcriptome of human PBMCs by the vitamin D metabolites 25-hydroxyvitamin D3 (25(OH)D3), 25(OH)D2 and 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3).
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| Overall design |
PBMCs of a healthy male donor were isolated within one hour after peripheral blood collection and treated for 4, 8 or 24 h either with 1,25(OH)2D3 (0.1, 1 and 10 nM), 25(OH)2D3 (100, 250, 500, 750 and 1000 nM), 25(OH)D2 (100, 250, 500, 750 and 1000 nM) or solvent (0.1% EtOH).
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| Web link |
https://www.frontiersin.org/articles/10.3389/fnut.2022.910601/full
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| Contributor(s) |
Hanel A, Carlberg C |
| Citation(s) |
35911100 |
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| Submission date |
Mar 23, 2022 |
| Last update date |
Aug 03, 2022 |
| Contact name |
Andrea Hanel |
| E-mail(s) |
[email protected], [email protected]
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| Organization name |
University of Eastern Finland
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| Department |
Institute of Biomedicine
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| Lab |
Prof. Carsten Carlberg Lab
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| Street address |
Yliopistonranta 1E, POB 1627
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| City |
Kuopio |
| ZIP/Postal code |
70211 |
| Country |
Finland |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (66)
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| GSM5968514 |
PBMC, 0.1% EtOH, 08 h, rep1 |
| GSM5968515 |
PBMC, 0.1% EtOH, 08 h, rep2 |
| GSM5968516 |
PBMC, 0.1% EtOH, 08 h, rep3 |
| GSM5968517 |
PBMC, 0.1% EtOH, 24 h, rep1 |
| GSM5968518 |
PBMC, 0.1% EtOH, 24 h, rep2 |
| GSM5968519 |
PBMC, 0.1% EtOH, 24 h, rep3 |
| GSM5968520 |
PBMC, 10 nM 1,25(OH)2D3, 04 h, rep1 |
| GSM5968521 |
PBMC, 10 nM 1,25(OH)2D3, 04 h, rep2 |
| GSM5968522 |
PBMC, 10 nM 1,25(OH)2D3, 04 h, rep3 |
| GSM5968523 |
PBMC, 10 nM 1,25(OH)2D3, 08 h, rep1 |
| GSM5968524 |
PBMC, 10 nM 1,25(OH)2D3, 08 h, rep2 |
| GSM5968525 |
PBMC, 10 nM 1,25(OH)2D3, 08 h, rep3 |
| GSM5968526 |
PBMC, 1000 nM 25(OH)D3, 04 h, rep1 |
| GSM5968527 |
PBMC, 1000 nM 25(OH)D3, 04 h, rep2 |
| GSM5968528 |
PBMC, 1000 nM 25(OH)D3, 04 h, rep3 |
| GSM5968529 |
PBMC, 1000 nM 25(OH)D3, 08 h, rep1 |
| GSM5968530 |
PBMC, 1000 nM 25(OH)D3, 08 h, rep2 |
| GSM5968531 |
PBMC, 1000 nM 25(OH)D3, 08 h, rep3 |
| GSM5968532 |
PBMC, 1000 nM 25(OH)D2, 04 h, rep1 |
| GSM5968533 |
PBMC, 1000 nM 25(OH)D2, 04 h, rep2 |
| GSM5968534 |
PBMC, 1000 nM 25(OH)D2, 04 h, rep3 |
| GSM5968535 |
PBMC, 1000 nM 25(OH)D2, 08 h, rep1 |
| GSM5968536 |
PBMC, 1000 nM 25(OH)D2, 08 h, rep2 |
| GSM5968537 |
PBMC, 1000 nM 25(OH)D2, 08 h, rep3 |
| GSM5968538 |
PBMC, 0.1 nM 1,25(OH)2D3, 24 h, rep1 |
| GSM5968539 |
PBMC, 0.1 nM 1,25(OH)2D3, 24 h, rep2 |
| GSM5968540 |
PBMC, 0.1 nM 1,25(OH)2D3, 24 h, rep3 |
| GSM5968541 |
PBMC, 1 nM 1,25(OH)2D3, 24 h, rep1 |
| GSM5968542 |
PBMC, 1 nM 1,25(OH)2D3, 24 h, rep2 |
| GSM5968543 |
PBMC, 1 nM 1,25(OH)2D3, 24 h, rep3 |
| GSM5968544 |
PBMC, 10 nM 1,25(OH)2D3, 24 h, rep1 |
| GSM5968545 |
PBMC, 10 nM 1,25(OH)2D3, 24 h, rep2 |
| GSM5968546 |
PBMC, 10 nM 1,25(OH)2D3, 24 h, rep3 |
| GSM5968547 |
PBMC, 100 nM 25(OH)D3, 24 h, rep1 |
| GSM5968548 |
PBMC, 100 nM 25(OH)D3, 24 h, rep2 |
| GSM5968549 |
PBMC, 100 nM 25(OH)D3, 24 h, rep3 |
| GSM5968550 |
PBMC, 250 nM 25(OH)D3, 24 h, rep1 |
| GSM5968551 |
PBMC, 250 nM 25(OH)D3, 24 h, rep2 |
| GSM5968552 |
PBMC, 250 nM 25(OH)D3, 24 h, rep3 |
| GSM5968553 |
PBMC, 500 nM 25(OH)D3, 24 h, rep1 |
| GSM5968554 |
PBMC, 500 nM 25(OH)D3, 24 h, rep2 |
| GSM5968555 |
PBMC, 500 nM 25(OH)D3, 24 h, rep3 |
| GSM5968556 |
PBMC, 750 nM 25(OH)D3, 24 h, rep1 |
| GSM5968557 |
PBMC, 750 nM 25(OH)D3, 24 h, rep2 |
| GSM5968558 |
PBMC, 750 nM 25(OH)D3, 24 h, rep3 |
| GSM5968559 |
PBMC, 1000 nM 25(OH)D3, 24 h, rep1 |
| GSM5968560 |
PBMC, 1000 nM 25(OH)D3, 24 h, rep2 |
| GSM5968561 |
PBMC, 1000 nM 25(OH)D3, 24 h, rep3 |
| GSM5968562 |
PBMC, 100 nM 25(OH)D2, 24 h, rep1 |
| GSM5968563 |
PBMC, 100 nM 25(OH)D2, 24 h, rep2 |
| GSM5968564 |
PBMC, 100 nM 25(OH)D2, 24 h, rep3 |
| GSM5968565 |
PBMC, 250 nM 25(OH)D2, 24 h, rep1 |
| GSM5968566 |
PBMC, 250 nM 25(OH)D2, 24 h, rep2 |
| GSM5968567 |
PBMC, 250 nM 25(OH)D2, 24 h, rep3 |
| GSM5968568 |
PBMC, 500 nM 25(OH)D2, 24 h, rep1 |
| GSM5968569 |
PBMC, 500 nM 25(OH)D2, 24 h, rep2 |
| GSM5968570 |
PBMC, 500 nM 25(OH)D2, 24 h, rep3 |
| GSM5968571 |
PBMC, 750 nM 25(OH)D2, 24 h, rep1 |
| GSM5968572 |
PBMC, 750 nM 25(OH)D2, 24 h, rep2 |
| GSM5968573 |
PBMC, 750 nM 25(OH)D2, 24 h, rep3 |
| GSM5968574 |
PBMC, 1000 nM 25(OH)D2, 24 h, rep1 |
| GSM5968575 |
PBMC, 1000 nM 25(OH)D2, 24 h, rep2 |
| GSM5968576 |
PBMC, 1000 nM 25(OH)D2, 24 h, rep3 |
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| Relations |
| BioProject |
PRJNA819096 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE199273_normalized_counts.xlsx |
12.4 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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