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| Status |
Public on Oct 27, 2022 |
| Title |
Alteration of global transcription by the artificial sweetener acesulfame K in Acinetobacter baumannii AB5075 |
| Organism |
Acinetobacter baumannii AB5075 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We perform RNA-seq comparing a treatment with 1.33% acesulfame K to a mock treatment in A. baumannii AB5075 in order to describe the alterations in global transcription produced by this artificial sweetener.
A. baumannii AB5075 cultures were grown in LB medium in the presence of 1.33% acesulfame K or a water control (3 biological replicates per condition) until reaching mid-exponential phase. At this point, cells were harvested, treated with RNAlate for preservation of total RNA and stored at -80 C. After that, total RNA was extracted from each sample.
As a result, 212 genes appeared upregulated in the presence of acesulfame K, whereas 252 were downregulated. In highlights, the gene functional groups showing the strongest transcriptional alterations were those related to twtching motility and natural transformation (downregulation of genes involves in biogenesis and control of Type IV pili and com genes), as well as genes involved in iron acquisition and siderophore biosynthesis and an uncharacterised gene cluster that might be involved in detoxification of sulfonamide compounds.
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| Overall design |
Three independent replicates of A. baumannii AB5075 were grown to mid-exponential phase in LB medium supplemented with 1.33% acesulfame K and compared to three independent replicates grown in the same conditions but receiving a mock treatment (equivalent volume of water).
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| Contributor(s) |
de Dios R, Proctor C, Maslova E, Dzalbe S, McCarthy RR |
| Citation(s) |
36412260 |
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| Submission date |
Mar 29, 2022 |
| Last update date |
Jan 25, 2023 |
| Contact name |
Rubén de Dios |
| E-mail(s) |
[email protected], [email protected]
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| Organization name |
Brunel University London
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| Department |
Life Sciences
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| Lab |
Ronan McCarthy lab (RMC)
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| Street address |
Kingston Lane
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| City |
Uxbridge |
| State/province |
Middlesex |
| ZIP/Postal code |
UB8 3PH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL32092 |
NextSeq 2000 (Acinetobacter baumannii AB5075) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA821209 |
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