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| Status |
Public on Apr 30, 2024 |
| Title |
Characterization of mast cells from human umbilical cord blood |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Mast cells are tissue-resident innate immune cells that express high affinity to receptor immunoglobulin E and responsible for host defense and array of diseases relate to immune system.
We aim to characterize the function of human umbilical cord blood-dervied mast cells (hCBMCs).
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| Overall design |
CD34+ cells were isolated from human umbilical cord blood and differentiated into mast cells using rhIL-6 and rh-SCF for 6-8 weeks. Total RNA was extracted from hCBMCs and CD34- cells and hybridized using Affymetrix Human Gene ST 1.0 arrays.
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| Contributor(s) |
Bakhashab S, Banafea GH, Ahmed F, Alsehli H, Alshaibi HF, Bagatian N, Subhi O, Gauthaman K, Rasool M, Schulten H, Pushparaj PN |
| Citation(s) |
37634863 |
| |
| Submission date |
Apr 25, 2022 |
| Last update date |
Jul 31, 2024 |
| Contact name |
Sherin Bakhashab |
| E-mail(s) |
[email protected]
|
| Organization name |
King Abdulaziz University
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| Department |
Biochemistry
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| Street address |
Abdullah Sulyman
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| City |
Jeddah |
| ZIP/Postal code |
21589 |
| Country |
Saudi Arabia |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA831842 |
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