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| Status |
Public on Feb 08, 2023 |
| Title |
Limosilactobacillus fermentum Limits Candida glabrata Growth by Ergosterol Depletion |
| Organism |
Nakaseomyces glabratus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Candida glabrata is a human-associated opportunistic fungal pathogen. It
shares its niche with Lactobacillus spp. in the gastrointestinal and vaginal tract. In fact, Lactobacillus species are thought to competitively prevent Candida overgrowth. We investigated the molecular aspects of this antifungal effect by analyzing the interaction of C. glabrata strains with Limosilactobacillus fermentum. From a collection of clinical C. glabrata isolates, we identified strains with different sensitivities to L. fermentum in coculture. We analyzed the variation of their expression pattern to isolate the specific response to L. fermentum. C. glabrata-L. fermentum coculture induced genes associated with ergosterol biosynthesis, weak acid stress, and drug/chemical stress. L. fermentum coculture depleted C. glabrata ergosterol. The reduction of ergosterol was dependent on the Lactobacillus species, even in coculture with different Candida species. We found a similar ergosterol-depleting effect with other lactobacillus strains (Lactobacillus crispatus and Lactobacillus rhamosus) on Candida albicans, Candida tropicalis, and Candida krusei. The addition of ergosterol improved C. glabrata growth in the coculture. Blocking ergosterol synthesis with fluconazole increased the susceptibility against L. fermentum, which was again mitigated by the addition of ergosterol. In accordance, a C. glabrata Derg11 mutant, defective in ergosterol biosynthesis, was highly sensitive to L. fermentum. In conclusion, our analysis indicates an unexpected direct function of ergosterol for C. glabrata proliferation in coculture with L. fermentum.
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| Overall design |
Differential gene expression profiles for four clincal Candida glabrata isolates (at least duplicates) in co-culture together with L. fermentum.
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| Contributor(s) |
Zangl I, Beyer R, Gattesco A, Labuda R, Pap I, Strauss J, Schüller C |
| Citation(s) |
36802215 |
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| Submission date |
May 10, 2022 |
| Last update date |
May 03, 2023 |
| Contact name |
Isabella Zangl |
| E-mail(s) |
[email protected]
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| Organization name |
University of Natural Resources and Life Sciences, Vienna
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| Department |
Insitute for Microbial Genetics
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| Street address |
Konrad Lorenz Str. 24
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| City |
Tulln an der Donau |
| ZIP/Postal code |
3430 |
| Country |
Austria |
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| Platforms (1) |
| GPL25492 |
Illumina HiSeq 2500 ([Candida] glabrata) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA836885 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE202656_raw_counts_bowtie.csv.gz |
233.1 Kb |
(ftp)(http) |
CSV |
| GSE202656_res122P_deseq2.csv.gz |
197.3 Kb |
(ftp)(http) |
CSV |
| GSE202656_res127P_deseq2.csv.gz |
198.1 Kb |
(ftp)(http) |
CSV |
| GSE202656_res132P_deseq2.csv.gz |
197.4 Kb |
(ftp)(http) |
CSV |
| GSE202656_resBG2_deseq2.csv.gz |
197.9 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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