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Series GSE20338 Query DataSets for GSE20338
Status Public on Jul 07, 2010
Title Candida albicans DOPA-grown cells
Organism Candida albicans
Experiment type Expression profiling by array
Summary The fungal pathogen Candida albicans produces dark-pigmented melanin when grown in a basal medium containing 1 mM l-DOPA as melanin substrate. In the widely used C. albicans strain SC5314, melanin appeared after 3-4 days of incubation in l-DOPA medium. The experiment was designed to reveal cadidate genes associated with melanin biosynthesis by expression profiling at different times of growth with and without L-DOPA added to the medium. Expression profiling of C. albicans revealed very few genes significantly up- or down-regulated by growth in l-DOPA.
 
Overall design Sixteen paired samples were submitted to dual channel analysis. Four biological replicate samples of RNA were prepared from cells of C. albicans strain SC5314 at four time points, from cultures grown with and without addition of 1 mM L-DOPA, thus generating an initial total of 32 samples for analysis: four experimental and four control samples made after 6 h, 24 h, 48 h and 72 h of growth. For each time point the control (no L-DOPA) samples were pooled and labelled with Cy3-dUTP by reverse transcription. The four individual biological replicates for each time point were separately labelled with Cy5-dUTP by reverse transcription and independently hybridized against the pooled controls on the microarray chips. Results were read with a ScanArray 4000 Microarray Analysis System.
 
Contributor(s) Odds FC
Citation(s) 20543065
Submission date Feb 16, 2010
Last update date Jan 18, 2013
Contact name Frank C Odds
E-mail(s) [email protected]
Organization name University of Aberdeen
Department School of Medical Sciences
Lab AFG
Street address Institute of Medical Sciences
City Aberdeen
ZIP/Postal code AB25 2ZD
Country United Kingdom
 
Platforms (1)
GPL7476 OPERON_AROSV1.1.2_Candida_albicans_array
Samples (16)
GSM509591 Cells + DOPA 6 h rep 1
GSM509592 Cells + DOPA 6 h rep 2
GSM509593 Cells + DOPA 6 h rep 3
Relations
BioProject PRJNA125411

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE20338_RAW.tar 9.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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