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| Status |
Public on Nov 19, 2022 |
| Title |
Enhanced myogenesis through LncFAM-mediated recruitment of HNRNPL to the MYBPC2 promoter I |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We identified a novel muscle-specific lncRNA, lncFAM71E1-2:2 (lncFAM), which increased robustly during early human myogenesis. Overexpression of lncFAM promoted differentiation of human myoblasts into myotubes, while silencing lncFAM suppressed this process. A nucleus-resident lncRNA, lncFAM was studied using chromatin isolation by RNA purification followed by mass spectrometry (ChIRP-ms) analysis to identify the molecular mechanisms whereby it might promote myogenesis. Analysis of lncFAM-interacting proteins revealed that lncFAM recruited the RNA-binding protein HNRNPL to the promoter of MYPBPC2, in turn increasing MYBPC2 mRNA transcription and enhancing production of the myogenic protein MYPBPC2. These results highlight a mechanism whereby a novel ribonucleoprotein complex, lncFAM-HNRNPL promotes MYBPC2 expression transcriptionally to increase myogenesis.
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| Overall design |
Examination of differentially expressed genes after silencing of lncFAM in human myoblasts.
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| Contributor(s) |
Chang MW, Yang JH, Yang X, Martindale JL, Munk R, Tsitsipatis D, Pandey PR, Piao Y, Mazan-Mamczarz K, De S, Abdelmohsen K, Wilson GM, Gorospe M |
| Citation(s) |
36533518 |
| |
| Submission date |
May 26, 2022 |
| Last update date |
Jan 25, 2023 |
| Contact name |
Supriyo De |
| Organization name |
NIA-IRP, NIH
|
| Department |
Laboratory of Genetics and Genomics
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| Lab |
Computational Biology & Genomics Core
|
| Street address |
251 Bayview Blvd
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE215343 |
Enhanced myogenesis through LncFAM-mediated recruitment of HNRNPL to the MYBPC2 promoter |
|
| Relations |
| BioProject |
PRJNA842674 |
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