|
| Status |
Public on Oct 29, 2024 |
| Title |
Obesity-instructed TREM2-high macrophages identified by comparative analysis of diabetic mouse and human kidney at single-cell resolution [scRNA-seq: DKD_mouse] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The cross-species molecular heterogeneity from mice to patients poses grand challenges for translational research endeavors. A high-fat diet (HFD) model and a genetic model (BTBR ob/ob) at different stand points were used to generate cell atlases of diabetic and obese mise.
We identified a previously unrecognized, expanding Trem2-High macrophage population in kidneys of HFD mice that modeled human TREM2-high macrophages in obese patients. Taken together, our cross-species comparison highlights shared immune and metabolic cell-state changes.
|
| |
|
| Overall design |
For the BTBR ob/ob genetic mouse model, we included n=5 and n=3 biological replicates for the 5- and 10-week time-point respectively for both ob/ob and wildtype strains. For the High-Fat diet (HFD) model, we combined n=4 biological replicates into a pooled “aged” group, in each of the chow and HFD conditions. The “aged” group ranged from 66 to 100 weeks old (schematic Figure 4A). For 3 biological replicates in each condition (Chow: ages 94,100; HFD: ages 83,100), there were 2 technical replicates or 10x channels run from the same cortical sample leading to a total of 7 libraries per condition.
|
| |
|
| Contributor(s) |
Subramanian A, Greka A |
| Citation(s) |
38781074 |
| |
| Submission date |
Jun 07, 2022 |
| Last update date |
Oct 30, 2024 |
| Contact name |
Ayshwarya Subramanian |
| E-mail(s) |
[email protected]
|
| Organization name |
Broad Institute
|
| Department |
Klarman Cell Observatory
|
| Street address |
415 Main Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (41)
|
|
| Relations |
| BioProject |
PRJNA846680 |
Less...
More...