|
| Status |
Public on Jun 24, 2022 |
| Title |
MraZ is a transcriptional inhibitor of cell division in Bacillus subtilis |
| Organism |
Bacillus subtilis PY79 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate the possible genes regulated by the DNA binding protein MraZ
The bacterial division and cell wall (dcw) cluster is a highly conserved region of the genome which encodes several essential cell division factors including the central divisome protein FtsZ. Understanding the regulation of this region is key to our overall understanding of the division process. mraZ is found at the 5’ end of the dcw cluster and previous studies have described MraZ as a sequence-specific DNA binding protein. In this article, we investigate MraZ to elucidate its role in Bacillus subtilis. Through our investigation, we demonstrate that increased levels of MraZ result in lethal filamentation due to repression of its own operon (mraZ-mraW-ftsL-pbpB). We observe rescue of filamentation upon decoupling ftsL expression, but not other genes in the operon, from MraZ control. Our data suggests that regulation of the mra operon may be an alternative way for cells to quickly arrest cytokinesis potentially during entry into stationary phase and in the event of DNA replication arrest. Furthermore, through timelapse microscopy we were able to identify that overexpression of mraZ or depletion of FtsL results in de-condensation of the FtsZ ring (Z-ring). Using fluorescent D-amino acid labelling, we also observed that coordinated peptidoglycan insertion at division site is dysregulated in the absence of FtsL. Thus, we reveal the precise role of FtsL is in Z-ring maturation and focusing septal peptidoglycan synthesis.
|
| |
|
| Overall design |
Comparative analysis of RNA-seq data of wild type B. subtilis (PY79), del-mraZ (MW192), or overexpression strains of mraZ (MW189) and mutant mraZ-R15A (MW256)
|
| |
|
| Contributor(s) |
Prahathees E |
| Citation(s) |
35943250 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R35 GM133617 |
Division site determination in Gram-positive bacteria |
UNIVERSITY OF SOUTH FLORIDA |
Prahathees Jai Eswara |
|
| |
| Submission date |
Jun 21, 2022 |
| Last update date |
Sep 23, 2022 |
| Contact name |
Prahathees Eswara |
| E-mail(s) |
[email protected]
|
| Organization name |
University of South Florida
|
| Street address |
4202 E Fowler Ave ISA2015
|
| City |
Tampa |
| State/province |
Florida |
| ZIP/Postal code |
33620 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL32384 |
NextSeq 2000 (Bacillus subtilis PY79) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA851398 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE206572_120_Differentially_Expressed_Genes_In_MW256vPY79.tsv.gz |
7.7 Kb |
(ftp)(http) |
TSV |
| GSE206572_150_Differentially_Expressed_Genes_In_MW192vPY79.tsv.gz |
9.5 Kb |
(ftp)(http) |
TSV |
| GSE206572_766_Differentially_Expressed_Genes_In_MW189vPY79.tsv.gz |
45.0 Kb |
(ftp)(http) |
TSV |
| GSE206572_MW189vPY79_All_Quantified_Genes.tsv.gz |
229.3 Kb |
(ftp)(http) |
TSV |
| GSE206572_MW192vPY79_All_Quantified_Genes.tsv.gz |
199.8 Kb |
(ftp)(http) |
TSV |
| GSE206572_MW256vPY79_All_Quantified_Genes.tsv.gz |
197.8 Kb |
(ftp)(http) |
TSV |
| GSE206572_alignment.tsv.gz |
207 b |
(ftp)(http) |
TSV |
| GSE206572_hisat2_counts.tsv.gz |
42.5 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
Less...
More...