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| Status |
Public on Aug 31, 2022 |
| Title |
Differential expression of cell wall remodeling genes is part of the dynamic phase-specific transcriptional program of conidial germination of Trichoderma asperelloides |
| Organism |
Trichoderma asperelloides |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The nature of saprophytic and mycoparasitic hyphal growth of Trichoderma spp. has been studied extensively, yet information concerning conidial germination in this genus is lacking. Using near-synchronous germinating cultures of Trichoderma asperelloides, we followed the morphological progression from dormant conidia to initial polar growth, to germling formation and to evidence for first branching. We found that the stage-specific transcriptional profile of T. asperelloides is one of the most dynamic described to date; Transcript abundance of over 5000 genes, comprising approximately half of the annotated genome, was uniquely reduced in the transition from dormancy to polar growth, providing a potentially high degree of flexibility for germination on diverse substrates. Conversely, after the onset of germination, transcript abundance of approximately a quarter of the genome was exclusively elevated during transition from elongation to initial branching. Bayesian network analysis has identified several chitinases (including chi42) and glucanase-encoding genes as hallmarks of transcriptional hubs during germination. Furthermore, the fact that the expression of specific members of the chitin synthase and glucan elongase families was significantly increased during germination in the presence of Rhizoctonia solani, a known host of the mycoparasite, indicates that host recognition can occur during early stages of mycoprasite development.
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| Overall design |
mRNA were sampled from 4 stages of Trichoderma asperelloides conidial germination on PDA and on Rhizoctonia solani living cultures
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| Contributor(s) |
Wang Z, Lopez-Giraldez F, Yarden O |
| Citation(s) |
36012842 |
| |
| Submission date |
Jun 27, 2022 |
| Last update date |
Aug 31, 2022 |
| Contact name |
Francesc Lopez |
| E-mail(s) |
[email protected]
|
| Organization name |
Yale University
|
| Department |
Department of Genetics
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| Lab |
YCGA
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| Street address |
P.O. Box 27386
|
| City |
West Haven |
| State/province |
CT |
| ZIP/Postal code |
06516 |
| Country |
USA |
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| Platforms (1) |
| GPL32402 |
Illumina HiSeq 2500 (Trichoderma asperelloides) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA853442 |
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