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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 18, 2010 |
| Title |
Expression data from effector CD4 T cells isolated from MRL/Faslpr mice. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
MRL/Faslpr mice is a lupus prone strain that exhibits lupus disease features at 12-16 weeks of age, including high-titer circulating anti-DNA antibodies, splenomegaly, lymphadnopathy, skin lesions, and IgG deposits in the kidney. At 16-24 weeks of age, CD4+ B220- CD44+ T cells were sorted into three populations based on the expression of two cell surface molecules, CD62L and PSGL1. CD62Lhi PSGL1hi, CD62Llo PSGL1hi, and CD62Llo PSGL1lo CD4+ T cells were isolated directly ex vivo. There was no treatment given to the animals. Naive (CD62Lhi CD44lo) CD4+ B220- T cells were isolated from young 6-8 week old female mice for comparison.
We used a microarray to identify unique features of the CD62Llo PSGL1lo population in comparison to naïve CD4+ T cells and other activated CD4+ T cells.
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| Overall design |
Cells were isolated from the spleens of aged (16-24 weeks) female MRL/Faslpr mice directy ex vivo, and immediatley sorted into 3 populations; CD62Lhi PSGL1hi, CD62Llo PSGL1hi, and CD62Llo PSGL1lo. All 3 populations of cells were previously gated on TCRb+, CD4+, B220-, CD44+. Naive CD4 T cells were isolated directly ex vivo from the spleens of young (6-8 weeks) female MRL/Faslpr mice, and immediately sorted by gating on TCRb+, CD4+, B220-, CD62Lhi, CD44lo. Three indepedent sorts were performed. RNA was isolated using Qiagen's RNAeasy kit and total RNA was submitted to the W.M. Keck Foundation Biotechnology Resource Laboratory at Yale for amplification and hybridization to Affymetrix Mouse Genome 430 2.0 GeneChips.
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| Contributor(s) |
Poholek AC, Craft J |
| Citation(s) |
20519643 |
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| Submission date |
Mar 10, 2010 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Amanda Poholek |
| E-mail(s) |
[email protected]
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| Organization name |
Yale University
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| Street address |
300 Cedar St. TAC S530
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| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06519 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| GSM520509 |
CD62Lhi PSGL1hi, technical replicate 1 |
| GSM520510 |
CD62Llo PSGL1hi, technical replicate 1 |
| GSM520511 |
CD62Llo PSGL1lo, technical replicate 1 |
| GSM520512 |
CD62Lhi PSGL1hi, technical replicate 2 |
| GSM520513 |
CD62Llo PSGL1hi, technical replicate 2 |
| GSM520514 |
CD62Llo PSGL1lo, technical replicate 2 |
| GSM520515 |
CD62Lhi PSGL1hi, technical replicate 3 |
| GSM520516 |
CD62Llo PSGL1hi, technical replicate 3 |
| GSM520517 |
CD62Llo PSGL1lo, technical replicate 3 |
| GSM520518 |
CD62Lhi, CD44lo technical replicate 1 |
| GSM520519 |
CD62Lhi, CD44lo technical replicate 2 |
| GSM520520 |
CD62Lhi, CD44lo technical replicate 3 |
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| Relations |
| BioProject |
PRJNA124971 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20735_RAW.tar |
49.5 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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