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| Status |
Public on Sep 14, 2023 |
| Title |
Bifidobacterium infantis intracellular 2-oxogluturate concentration is inversely related to nitrogen accessibility |
| Organism |
Bifidobacterium longum subsp. infantis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Bifidobacterium longum subsp. infantis (B. infantis) resides in the human infant gut and helps with the utilization of human milk-derived nutrient components. While its utilization of various carbohydrate sources has been studied extensively, mechanisms behind utilization of nitrogen components from human milk remain largely unknown. In this study, we present B. infantis growth profiles on the N-containing human milk oligosaccharides (HMO) as nitrogen sources, namely, lacto-N-tetraose (LNT) and lacto-N-neotetraose (LNnT). Dietary 2-Oxoglutarate (2-OG) in known in mice model for its protective effects against intestinal inflammation and colitis development. In this study, we have shown that B. infantis had increased 2-OG concentration when utilizes LNT or LNnT as a primary nitrogen source. As LNT and LNnT are the isomers of HMO core structures, N-acetyl glucosamine (NAG), the N-containing monosaccharide, was regarded as the nitrogen provider of the HMO core structures. Differentially expressed gene patterns in B. infantis were analyzed under the less efficient nitrogen conditions (HMOs and NAG) relative to the complex nitrogen controls. Proteomics analysis of B. infantis using 15N-labeled NAG revealed that NAG nitrogen was incorporated into B. infantis metabolism. Transcriptomics results of B. infantis in LNT, LNnT and NAG nitrogen were consistent with the proteomics results. This further indicated that B. infantis metabolism was affected by NAG nitrogen in nitrogen assimilation, HMO catabolism, NAD cofactor biosynthesis and regeneration, and peptidoglycan biosynthesis pathways. In summary, B. infantis can use NAG-containing HMO as a nitrogen source and incorporate NAG nitrogen into metabolism pathways.
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| Overall design |
Gene expression of B. infantis ATCC 15697 while growing on NAG, LNT, LNnT, pooled HMO, and complex nitrogen. Gene expression of B. infantis JCM 1260, B. infantis JCM 7009 while growing on NAG as a nitrogen source.
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| Contributor(s) |
Li S, You X, Rani A, Ozcan E, Sela DA |
| Citation(s) |
37609905 |
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| Submission date |
Jul 25, 2022 |
| Last update date |
Sep 14, 2023 |
| Contact name |
Shuqi Li |
| Organization name |
Harvard Medical School and Brigham and Women's Hospital
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| Department |
Neurology
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| Lab |
Cox Lab
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| Street address |
60 Fenwood Rd.
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL28926 |
Illumina NextSeq 500 (Bifidobacterium longum subsp. infantis) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA862067 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE209665_DESeq2-UMA272.299.302nagctrl.nagcys.summary.csv.gz |
293.8 Kb |
(ftp)(http) |
CSV |
| GSE209665_UMA272_HMO_as_N.txt.gz |
30.9 Kb |
(ftp)(http) |
TXT |
| GSE209665_UMA272_UMA299_UMA302_NAG_as_N_feature_table.txt.gz |
40.8 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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