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| Status |
Public on Nov 30, 2022 |
| Title |
Effect of endothelial-specific endorepellin expression on gene expression of breast cancer allografts in Tie2CreER;ERKi mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To explore the translational implications of endorepellin in breast cancer, we created a double transgenic, inducible Tie2CreER;ERKi mouse line that expresses recombinant endorepellin specifically from the endothelium. We investigated the therapeutic effects of recombinant endorepellin overexpression in an orthotopic, syngeneic breast cancer allograft mouse model.
Tamoxifen-induced expression of recombinant endorepellin specifically from the endothelium in Tie2CreER;ERKi mice markedly suppressed breast cancer allograft growth, hyaluronan deposition in the tumor proper and perivascular tissues, and tumor angiogenesis.
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| Overall design |
To investigate the effects of endorepellin in the vasculature of triple-negative breast cancer in mice, we generated double transgenic mice expressing a tamoxifen-inducible endothelial-specific endorepellin knock-in gene, injected mice with either tamoxifen or control, and then injected syngeneic mouse breast cancer cells into the mammary fat pads. Breast tumor allografts were harvested at 22 days, RNA was extracted from tumor samples and sent for RNAseq analysis.
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| Web link |
https://doi.org/10.1016/j.matbio.2023.03.005
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| Contributor(s) |
Iozzo RV, Chen CG |
| Citation(s) |
36907428 |
| |
| Submission date |
Jul 25, 2022 |
| Last update date |
Mar 21, 2023 |
| Contact name |
Renato V. Iozzo |
| E-mail(s) |
[email protected]
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| Organization name |
Thomas Jefferson University
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| Street address |
1020 Locust Street
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19107 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA862283 |
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