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| Status |
Public on Jun 30, 2024 |
| Title |
Transcriptome analysis in B16F10 and 4T1 cell lines with decitabine treatment [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goal of RNA-seq is to identify the differential expressed genes in the B16F10 and 4T1 cells after Decitabine treatment. Three biological replicates were assigned for each group and in total 12 groups were prepared for RNA-seq libraries with 300 ng mRNA as starting materials using NEXTflex Illumina qRNA-Seq Library Prep Kit (Bioo Scientific). We mapped over 60 million reads per sample to mouse genome (mm10) with RSEM workflow. P-values of differential expression were adjusted for multiple-hypothesis testing using False Discovery Rate (FDR) method.
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| Overall design |
Messenger RNA from B16F10 and 4T1 cell lines were extacted and cDNA libraries were generated for deep sequencing, in triplicate, using HiSeq 4000 or NovaSeq S4
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| Contributor(s) |
Zhang Y, Wang SY |
| Citation(s) |
39169233 |
| |
| Submission date |
Aug 25, 2022 |
| Last update date |
Sep 30, 2024 |
| Contact name |
Eric Greer |
| E-mail(s) |
[email protected]
|
| Organization name |
Boston Children's Hospital/Harvard Medical School
|
| Department |
Newborn Medicine/Department of Pediatrics
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| Lab |
Greer Lab
|
| Street address |
320 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (2) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE212029 |
B16 and 4T1 cell lines with decitabine treatment |
|
| Relations |
| BioProject |
PRJNA873589 |
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