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| Status |
Public on Feb 10, 2023 |
| Title |
Hodgkin lymphoma cell lines and tissues express mGluR5: a potential link to Ophelia syndrome and paraneoplastic neurological disease |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Ophelia syndrome is characterized by the coincidence of severe neuropsychiatric symptoms, classical Hodgkin lymphoma, and the presence of antibodies to the metabotropic glutamate 5 receptor (mGluR5). Little is known about the pathogenetic link between these symptoms and the role anti-mGluR5-antibodies play. We investigated lymphoma tissue from patients with Ophelia syndrome and with isolated classical Hodgkin lymphoma by quantitative immunocytochemistry for mGluR5-expression. Further, we studied the L-1236, L-428, L-540, SUP-HD1, KM-H2, and HDLM-2 classical Hodgkin lymphoma cell lines by FACS and Western blot for mGluR5-expression, and by transcriptome analysis. mGluR5 surface expression differed significantly in terms of receptor density, distribution pattern, and percentage of positive cells. Highest levels were found in the L-1236 line. RNA-sequencing revealed more than 800 genes that were higher expressed in L-1236 in comparison to classical Hodgkin lymphoma-controls. High mGluR5-expression was associated with upregulation of PI3K/AKT and MAPK pathways and of downstream targets (e.g. EGR1) known to be involved in classical Hodgkin lymphoma progression. Finally, mGluR5 expression was increased in the classical Hodgkin lymphoma-tissue of our Ophelia syndrome patient in contrast to five classical Hodgkin lymphoma-patients without autoimmune encephalitis. Given the association of encephalitis and classical Hodgkin’s lymphoma in Ophelia syndrome, it is possible that mGluR5-expression on classical Hodgkin lymphoma cells not only drives tumor progression, but may also trigger anti-mGluR5 encephalitis already before classical Hodgkin lymphoma-manifestation.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for 6 classical Hodgkin Lymphoma cell lines: L-1236, L-428, L-540, SUP-HD1, KM-H2, HDLM-2
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| Web link |
https://doi.org/10.3390/cells12040606
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| Contributor(s) |
Schuelke M, Nikolaus M |
| Citation(s) |
36831273 |
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| Submission date |
Aug 30, 2022 |
| Last update date |
Mar 09, 2023 |
| Contact name |
Markus Schuelke |
| E-mail(s) |
[email protected]
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| Phone |
++49 30 4505 66112
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| Organization name |
Charite
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| Department |
Neuropediatrics
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| Lab |
Schuelke lab
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| Street address |
Augustenburger Platz 1
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| City |
Berlin |
| State/province |
Berlin |
| ZIP/Postal code |
13353 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA875051 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE212326_RAW.tar |
8.3 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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