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| Status |
Public on Dec 13, 2022 |
| Title |
Transcriptome-wide analysis of pseudouridinylation in Drosophila melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
Pseudouridine (Psi) is one of the most frequent post-transcriptional modification of RNA. Enzymatic Psi modification occurs on rRNA, snRNA, snoRNA, tRNA, non-coding RNA and has recently been discovered on mRNA. Transcriptome-wide detection of Psi (Psi-seq) has yet to be performed for the widely studied model organism Drosophila melanogaster. Here, we optimized Psi-seq analysis for this species and have identified thousands of Psi modifications throughout the female fly head transcriptome. We find that Psi is widespread on both cellular and mitochondrial rRNAs. In addition, more than a thousand Psi sites were found on mRNAs. When pseudouridinylated, mRNAs frequently had many Psi sites. Many mRNA Psi sites are present in genes encoding for ribosomal proteins, and many are found in mitochondrial encoded RNAs, further implicating the importance of pseudouridinylation for ribosome and mitochondrial function. The 7SLRNA of the signal recognition particle is the non-coding RNA most enriched for Psi. The three mRNAs most enriched for Psi encode highly-expressed yolk proteins (YP1, YP2, YP3). By comparing the pseudouridine profiles in the RluA-2 mutant and the w1118 control genotype, we identified Psi sites that were missing in the mutant RNA as potential RluA-2 targets. Finally, differential gene expression analysis of the mutant transcriptome indicates a major impact of loss of RluA-2 on the ribosome and translational machinery.
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| Overall design |
Polyadenylated RNA was enriched from total RNA using two rounds of Oligo(dT) dynabeads (Invitrogen) according to manufacturer’s protocol. To each polyA-RNA sample, spike-in RNA (~3ug) was added and then the samples were split into two equal samples for CMCT or mock treatment.
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| Contributor(s) |
Song W, Podicheti R, Rusch DB, Tracey WD |
| Citation(s) |
36534986 |
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| Submission date |
Sep 14, 2022 |
| Last update date |
Mar 15, 2023 |
| Contact name |
W. Daniel Tracey |
| E-mail(s) |
[email protected]
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| Organization name |
Indiana University
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| Department |
Biology
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| Street address |
702 N. Walnut Grove
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| City |
Bloomington |
| State/province |
Indiana |
| ZIP/Postal code |
47401 |
| Country |
USA |
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| Platforms (1) |
| GPL22106 |
NextSeq 550 (Drosophila melanogaster) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA880512 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE213312_RAW.tar |
771.7 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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