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| Status |
Public on Sep 01, 2023 |
| Title |
Comparative analysis of sperm DNA methylation supports evolutionary acquired epigenetic plasticity controlling organ speciation |
| Organism |
Sus scrofa |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Gametic DNA methylation is essential to the viability of the embryo and participates in developmental programming. Here, we performed a comparative epigenomic analysis of DNA methylation in spermatozoa from humans, mice, rats and mini-pigs to get insight into species specificity of the gametic epigenetic blueprint. Using data from whole-genome bisulfite sequencing (WGBS) and reduced representation bisulfite sequencing (RRBS), we compared the methylation profiles of orthologous single-CpG sites. Transcription profiles of early embryo development were analyzed to provide insight into the association between sperm methylation and gene expression programming. We identified DNA methylation variation near genes related to the central nervous system and signal transduction. Gene expression dynamics at different time-points of preimplantation stages were modestly associated with spermatozoal DNA methylation at nearest promoters. Our analysis revealed that conserved genomic regions subject to epigenetic variation across different species were associated with specific organ functions, suggesting their potential contribution to organ speciation and long-term adaptation to the environment
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| Overall design |
We performed our analyses on a panel of publically available datasets, generated for different studies. In addition, we performed RRBS on mini-pigs sperm. Sperm samples were collected from four adult mini-pigs.
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| Web link |
https://www.futuremedicine.com/doi/10.2217/epi-2022-0168
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| Contributor(s) |
Moharrek F, Ingerslev LR, Altıntaş A, Lundell L, Hansen AN, Small L, Workman C, Barrès R |
| Citation(s) |
29087470 |
| |
| Submission date |
Sep 16, 2022 |
| Last update date |
Dec 08, 2023 |
| Contact name |
Farideh Moharrek |
| E-mail(s) |
[email protected]
|
| Organization name |
Novo Nordisk Foundation Center for Basic Metabolic Research
|
| Street address |
Hvidovrevej 22B
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| City |
Rodovre |
| ZIP/Postal code |
2610 |
| Country |
Denmark |
| |
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| Platforms (1) |
| GPL26351 |
Illumina NovaSeq 6000 (Sus scrofa) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA881317 |
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