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| Status |
Public on Jul 12, 2023 |
| Title |
Transcription and DNA methylation signatures of paternal care in hippocampal dentate gyrus of prairie voles (RRBS) |
| Organism |
Microtus ochrogaster |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
In socially monogamous prairie voles (Microtus ochrogaster), parental behaviors not only occur in mothers and fathers, but also can exist in virgin males. However, some virgin males display aggressive behaviors towards conspecific pups. Although this behavioral dichotomy in response to pup exposure has been well documented in male virgin voles, little is known about the gene expression changes underlie the parental behavioral differences and their regulatory mechanisms. To address this, we profiled the transcriptome and DNA methylome of hippocampal dentate gyrus of four prairie vole groups, attacker virgin males, parental virgin males, fathers, and mothers.
We found a concordant pattern of gene transcription in parental virgin males and fathers, when comparing to the attacker group. The methylome analysis also revealed pathways with genes enriched for epigenetic changes involving both receptor-mediated and secondary messenger signaling across both behavioral phenotypes and sexual experiences. Furthermore, we found correlations between gene expression changes and DNA methylation differences between attacker and parental virgin males, which suggests a canonical gene expression regulatory role of DNA methylation in paternal care. Therefore, our study presents an integrated view of transcriptome and epigenome that provides a DNA epigenetic based molecular insight of paternal behavior.
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| Overall design |
Comparative Gene Expression and DNA Methylome profiling of Prairie Vole Dentate Gyrus cells from different groups based on behavioral response to pup-exposure
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| Contributor(s) |
Waddell NJ, Liu Y, Chitaman JM, Kaplan GJ, Wang Z, Feng J |
| Citation(s) |
37419920 |
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| Submission date |
Oct 04, 2022 |
| Last update date |
Jul 13, 2023 |
| Contact name |
Nicholas James Waddell |
| E-mail(s) |
[email protected]
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| Organization name |
Florida State University
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| Department |
Biological Sciences
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| Lab |
Laboratory of Jian Feng
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| Street address |
319 Stadium Dr
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| City |
Tallahassee |
| State/province |
FL |
| ZIP/Postal code |
32306 |
| Country |
USA |
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| Platforms (1) |
| GPL28499 |
Illumina NovaSeq 6000 (Microtus ochrogaster) |
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| Samples (23)
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| GSM6616074 |
RRBS Dentate Gyrus cells, Parental Group, Rep 1 |
| GSM6616075 |
RRBS Dentate Gyrus cells, Parental Group, Rep 2 |
| GSM6616076 |
RRBS Dentate Gyrus cells, Parental Group, Rep 3 |
| GSM6616077 |
RRBS Dentate Gyrus cells, Parental Group, Rep 4 |
| GSM6616078 |
RRBS Dentate Gyrus cells, Parental Group, Rep 5 |
| GSM6616079 |
RRBS Dentate Gyrus cells, Parental Group, Rep 6 |
| GSM6616080 |
RRBS Dentate Gyrus cells, Attacker Group, Rep 1 |
| GSM6616081 |
RRBS Dentate Gyrus cells, Attacker Group, Rep 2 |
| GSM6616082 |
RRBS Dentate Gyrus cells, Attacker Group, Rep 3 |
| GSM6616083 |
RRBS Dentate Gyrus cells, Attacker Group, Rep 4 |
| GSM6616084 |
RRBS Dentate Gyrus cells, Attacker Group, Rep 5 |
| GSM6616085 |
RRBS Dentate Gyrus cells, Attacker Group, Rep 6 |
| GSM6616086 |
RRBS Dentate Gyrus cells, Father Group, Rep 1 |
| GSM6616087 |
RRBS Dentate Gyrus cells, Father Group, Rep 2 |
| GSM6616088 |
RRBS Dentate Gyrus cells, Father Group, Rep 3 |
| GSM6616089 |
RRBS Dentate Gyrus cells, Father Group, Rep 4 |
| GSM6616090 |
RRBS Dentate Gyrus cells, Father Group, Rep 5 |
| GSM6616091 |
RRBS Dentate Gyrus cells, Mother Group, Rep 1 |
| GSM6616092 |
RRBS Dentate Gyrus cells, Mother Group, Rep 2 |
| GSM6616093 |
RRBS Dentate Gyrus cells, Mother Group, Rep 3 |
| GSM6616094 |
RRBS Dentate Gyrus cells, Mother Group, Rep 4 |
| GSM6616095 |
RRBS Dentate Gyrus cells, Mother Group, Rep 5 |
| GSM6616096 |
RRBS Dentate Gyrus cells, Mother Group, Rep 6 |
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| This SubSeries is part of SuperSeries: |
| GSE214799 |
Transcription and DNA methylation signatures of paternal care in hippocampal dentate gyrus of prairie voles |
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| Relations |
| BioProject |
PRJNA887095 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE214798_RAW.tar |
176.1 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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