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| Status |
Public on Jul 08, 2024 |
| Title |
Protein aggregation in plant mitochondria lacking Lon1 inhibits translation and induces unfolded protein responses |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Loss of Lon1 led to stunted plant growth and accumulation of nuclear‐encoded mitochondrial proteins including Lon1 substrates. However, an in‐depth label‐free proteomics quantification of mitochondrial proteins in lon1 revealed that the majority of mitochondrial‐encoded proteins decreased in abundance. Additionally, we found that lon1 mutants contained protein aggregates in the mitochondrial that were enriched in metabolic enzymes, ribosomal subunits and PPR‐containing proteins of the translation apparatus. These mutants exhibited reduced general mitochondrial translation as well as deficiencies in RNA splicing and editing. These findings support the role of Lon1 in maintaining a functional translational apparatus for mitochondrial‐encoded gene translation. Transcriptome analysis of lon1 revealed a mitochondrial unfolded protein response reminiscent of the mitochondrial retrograde signalling dependent on the transcription factor ANAC017. Notably, lon1 mutants exhibited transiently elevated ethylene production, and the shortened hypocotyl observed in lon1 mutants during skotomorphogenesis was partially alleviated by ethylene inhibitors. Furthermore, the short root phenotype was partially ameliorated by introducing a mutation in the ethylene receptor ETR1. Interestingly, the upregulation of only a select few target genes was linked to ETR1‐mediated ethylene signalling. Together this provides multiple steps in the link between loss of Lon1 and signalling responses to restore mitochondrial protein homoeostasis in plants.
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| Overall design |
Arabidopsis thaliana is Columbia-0 (Col-0) background. T-DNA insertion mutant lon1-2 (AT5G26860, SALK_012797) are owned by our laboratory. Arabidopsis grown best under 16/8 hours light / dark cycle at room temperature (18-22 ° C), with light intensity of 120-150 µ mol / m2 (normal conditions). Seven-day-old seedlings were harvested in biological triplicate under normal conditions. Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer’s protocol. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The samples with RNA Integrity Number (RIN) ≥ 7 were subjected to the subsequent analysis. The libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions. Then these libraries were sequenced on the Illumina sequencing platform (DNBSEQ-T7) and 125bp/150bp paired-end reads were generated.
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| Web link |
http://10.1111/pce.15035
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| Contributor(s) |
Song C, Li L |
| Citation(s) |
38988259 |
| BioProject |
PRJNA803036 |
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| Submission date |
Oct 25, 2022 |
| Last update date |
Dec 31, 2024 |
| Contact name |
Ce Song |
| E-mail(s) |
[email protected]
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| Organization name |
Nankai University
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| Street address |
Weijin road NO.94
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| City |
Tianjin |
| ZIP/Postal code |
300000 |
| Country |
China |
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| Platforms (1) |
| GPL31102 |
DNBSEQ-T7 (Arabidopsis thaliana) |
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| Samples (6)
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE216535_RAW.tar |
1.9 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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