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| Status |
Public on Oct 31, 2022 |
| Title |
Transcriptome and metabolome analysis reveals anthocyanin biosynthesis pathway associated with poplar (Populus deltoids) leaf color formation |
| Organism |
Populus deltoides |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Colored-leaf plants are increasingly popular and have been attracting more and more attentions. However, studies about the anthocyanin components and molecular mechanisms of anthocyanin biosynthesis in QHP and ZSY was still unclear. In present study, an integrated metabolite and transcriptome profiling analysis was performed to explore the anthocyanin compositions and the specific regulatory network of anthocyanin biosynthesis in purple leaves of QHP and ZSY. A total of 39 anthocyanin compounds were detected based on the LC-MS/MS analysis. Of these, 12 cyanidins, 7 pelargonidins, 5 delphindins, and 5 procyanidins are the major anthocyanin compounds, which were significantly differentially accumulated in purple leaves of QHP and ZSY. The major genes associated with anthocyanin biosynthesis, including structural genes and TFs, were differentially expressed in the purple leaves of QHP and ZSY through RNA-seq data analysis, some of which were further assessed by qRT-PCR. Correlation between RNA-seq analysis and metabolite profiling showed that the expression pattern of some differentially expressed genes in anthocyanin biosynthes pathway were closely correlated with the differential accumulation of anthocyanins. In addition, one member of SG5 subgroup of R2R3-MYB TFs, Podel.04G021100, shows a similar expression pattern to some structure genes and closely correlates with sixteen anthocyanin compounds, indicating that the MBY TF (Podel.04G021100) may be involved in the regulation of anthocyanin biosynthesis. The above results not only make us systematic and comprehensive understand the anthocyanin components and corresponding molecular mechanisms of anthocyanin biosynthesis in purple leaves of QHP and ZSY, but also contribute to the promotion and application of anthocyanins in colored-leaf poplars.
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| Overall design |
In present study, an integrated metabolite and transcriptome profiling analysis was performed to explore the anthocyanin compositions and the specific regulatory network of anthocyanin biosynthesis in purple leaves of QHP and ZSY. A total of 39 anthocyanin compounds were detected based on the LC-MS/MS analysis. Of these, 12 cyanidins, 7 pelargonidins, 5 delphindins, and 5 procyanidins are the major anthocyanin compounds, which were significantly differentially accumulated in purple leaves of QHP and ZSY. The major genes associated with anthocyanin biosynthesis, including structural genes and TFs, were differentially expressed in the purple leaves of QHP and ZSY through RNA-seq data analysis, some of which were further assessed by qRT-PCR. Correlation between RNA-seq analysis and metabolite profiling showed that the expression pattern of some differentially expressed genes in anthocyanin biosynthes pathway were closely correlated with the differential accumulation of anthocyanins. In addition, one member of SG5 subgroup of R2R3-MYB TFs, Podel.04G021100, shows a similar expression pattern to some structure genes and closely correlates with sixteen anthocyanin compounds, indicating that the MBY TF (Podel.04G021100) may be involved in the regulation of anthocyanin biosynthesis.
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| Contributor(s) |
Peng X, Ai Y, Wang X, Zhuang W |
| Citation(s) |
36909390 |
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| Submission date |
Oct 28, 2022 |
| Last update date |
Jun 23, 2023 |
| Contact name |
yunfeng wei |
| E-mail(s) |
[email protected]
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| Phone |
14785897041
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| Organization name |
Guizhou University
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| Street address |
Huaxi District, Guiyang City
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| City |
Guiyang |
| ZIP/Postal code |
550000 |
| Country |
China |
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| Platforms (1) |
| GPL32787 |
Illumina NovaSeq 6000 (Populus deltoides) |
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| Samples (12)
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| GSM6690504 |
‘Zhongshanyuan poplar’（ZSY)，green-leaves 1 |
| GSM6690505 |
‘Zhongshanyuan poplar’（ZSY)，green-leaves 2 |
| GSM6690506 |
‘Zhongshanyuan poplar’（ZSY)，green-leaves 3 |
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| Relations |
| BioProject |
PRJNA895328 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE216754_RAW.tar |
4.2 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE216754_genome.fa.gz |
123.9 Mb |
(ftp)(http) |
FA |
| GSE216754_genome.gtf.gz |
5.5 Mb |
(ftp)(http) |
GTF |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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