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| Status |
Public on Feb 07, 2023 |
| Title |
Decapping factor Dcp2 controls mRNA abundance and translation to adjust metabolism and filamentation to nutrient availability |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Degradation of most yeast mRNAs involves decapping by Dcp1/Dcp2. DEAD-box protein Dhh1 has been implicated as an activator of decapping, and as a translational repressor, but their functions in cells are incompletely understood. We have analyzed these questions by a combination of ribosome profiling, RNA-Seq, CAGE analysis of capped mRNAs.
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| Overall design |
This study includes total 24 samples, 6 for ribosome profiling and 6 for RNA-Seq derived from duplicates of WT, dcp2D and dcp2-EE. Separately, total RNA of WT, dcp2, and xrn1 in duplicates, were subjected to CAGE-Seq (6 samples) and total RNA-Seq (6 samples). All strains were grown in YPD at 30ºC till the OD600 ~0.6
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| Contributor(s) |
Kumar Vijjamarri A, Niu X, Vandermeulen MD, Onu C, Zhang F, Qui H, Gupta N, Gaikwad S, Greenberg ML, Cullen PJ, Lin ZG, Hinnebusch AG |
| Citation(s) |
36711592, 37266577, 37439347 |
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| Submission date |
Oct 28, 2022 |
| Last update date |
Sep 27, 2023 |
| Contact name |
Alan G Hinnebusch |
| E-mail(s) |
[email protected]
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| Organization name |
National Institutes of Health
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| Department |
Eunice Kennedy Shriver National Institute of Child Health and Human Development
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| Lab |
Section on Nutrient Control of Gene Expression
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| Street address |
6 Center Drive
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| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (2) |
| GPL17342 |
Illumina HiSeq 2500 (Saccharomyces cerevisiae) |
| GPL19756 |
Illumina NextSeq 500 (Saccharomyces cerevisiae) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA895461 |
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