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| Status |
Public on May 31, 2023 |
| Title |
Decoding the transcriptome of muscular dystrophy using single-nucleus RNA sequencing |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Muscular dystrophy is a group of diseases that cause progressive weakness and degeneration of the skeletal muscles that control movement. Lacking polymerase I transcription release factor (PTRF, also known as Cavin1), an essential caveolae component, causes a secondary deficiency of caveolins resulting in muscular dystrophy. Because skeletal muscle is a heterogeneous tissue composed of different metabolic muscle fiber (myofibers) and mononuclear cells, the transcriptome responses of these myofibers and mononuclear cell to muscular dystrophy caused by PTRF deletion has not been explored. Here, we create muscular dystrophy mice caused by the deletion of PTRF gene and apply single-nucleus RNA sequencing (snRNA-seq) to unveil transcriptional changes in the skeletal muscle of mice with muscular dystrophy at single-nucleus resolution.
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| Overall design |
We analyzed quadriceps muscles from 3-month-old WT and PTRF KO mice using single-nucleus sequencing.
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| Web link |
https://faseb.onlinelibrary.wiley.com/doi/full/10.1096/fj.202201949RR
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| Contributor(s) |
Li X, Long K, Zhang R, Zhang J |
| Citation(s) |
37235502 |
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| Submission date |
Nov 06, 2022 |
| Last update date |
Aug 31, 2023 |
| Contact name |
Xiaokai Li |
| E-mail(s) |
[email protected]
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| Organization name |
Sichuan Agricultural University
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| Street address |
Huiming road #211
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| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
611130 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA898787 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE217390_RAW.tar |
48.6 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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