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| Status |
Public on Nov 10, 2010 |
| Title |
To determine the global gene expression response in murine LPS-induced macrophages treated with a bromodomain inhibitor. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Bone marrow-derived macrophages, Unstimulated DMSO Bone marrow-derived macrophages, Unstimulated + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 1h DMSO Bone marrow-derived macrophages, LPS 1h + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 2h DMSO Bone marrow-derived macrophages, LPS 2h + I-BET (GSK525762A) Bone marrow-derived macrophages, LPS 4h DMSO Bone marrow-derived macrophages, LPS 4h + I-BET (GSK525762A)
LPS (100 ng/mL) was purchased from Sigma. Bone Marrow-derived macrophages (BMDMs) were differentiated from C57BL/6 bone marrow using 5 ng/mL each of recombinant M-CSF and IL-3 (Peprotech) for 7 days as described (Jeffrey et al, Nature Immunology, 2006). 2 x10^6 BMDMs were treated with DMSO or 1 μM of I-BET for 30 minutes before the addition of LPS (100 ng/mL) for 1, 2 or 4h. Unstimulated control samples were incubated with I-BET only for 1 hour. 500 ng of total RNA from 3 independent samples per group was used to prepare biotin-labeled RNA using Ambion Illumina TotalPrep RNA Amplification Kit (Applied Biosystems) and hybridized to Illumina MouseRef-8 v2.0 expression BeadChip kits. The chips were scanned using Illumina BeadArray Reader.
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| Overall design |
3 biological replicates and 4 timepoints
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| Contributor(s) |
Jeffrey K, Tarakhovsky A |
| Citation(s) |
21068722 |
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| Submission date |
May 10, 2010 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Paul A Wilson |
| E-mail(s) |
[email protected]
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| Organization name |
GSK
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| Department |
Target Sciences
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| Lab |
Computational Biology
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| Street address |
GlaxoSmithKline Medicine Research Centre
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| City |
Stevenage |
| State/province |
Hertfordshire |
| ZIP/Postal code |
SG1 2NY |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA127281 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21764_RAW.tar |
3.1 Mb |
(http)(custom) |
TAR |
| GSE21764_non-normalized.txt.gz |
2.8 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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