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| Status |
Public on Feb 08, 2023 |
| Title |
Unraveling the Gene Regulatory Networks of the Global Regulators VeA and LaeA in Aspergillus nidulans [RNA-seq] |
| Organism |
Aspergillus nidulans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In the filamentous fungus Aspergillus nidulans, the velvet family protein VeA and the global regulator of secondary metabolism LaeA govern fungal development and secondary metabolism mostly by acting as the VelB/VeA/LaeA heterotrimeric complex. While functions of these highly conserved controllers have been well studied, the genome-wide regulatory networks governing cellular and chemical development remain to be uncovered. Here, by integrating transcriptomic analyses, protein-DNA interactions, and the known A. nidulans gene/protein interaction data, we have unraveled the gene regulatory networks governed by VeA and LaeA. Within the networks, VeA and LaeA directly control the expression of numerous genes involved in asexual/sexual development and primary/secondary metabolism in A. nidulans. Totals of 3,190 and 1,834 potential direct target genes of VeA and LaeA were identified, respectively, including several important developmental and metabolic regulators such as flbA·B·C, velB·C, areA, mpkB, and hogA. Moreover, by analyzing over 8,800 ChIP-seq peaks, we have revealed the predicted common consensus sequences 5’-TGATTGGCTG-3’ and 5’-TCACGTGAC-3’ that VeA and LaeA might bind to interchangeably. These findings further expand the biochemical and genomic studies of the VelB/VeA/LaeA complex functionality in gene regulation. In summary, this study unveils genes that are under the regulation of VeA and LaeA, proposes the VeA- and LaeA-mediated gene regulatory networks, and demonstrates their genome-wide developmental and metabolic regulations in A. nidulans. This entry is for the RNA-seq data.
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| Overall design |
Comparative gene expression profiling analysis by high throughput sequencing for wild-type, veA-deletion, and laeA-deletion strains.
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| Contributor(s) |
Moon H, Lee M, Bok I, Bok JW, Keller N, Yu J |
| Citation(s) |
36920196 |
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| Submission date |
Nov 11, 2022 |
| Last update date |
May 03, 2023 |
| Contact name |
Heungyun Harrison Moon |
| E-mail(s) |
[email protected]
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| Organization name |
University of Wisconsin-Madison
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| Department |
Bacteriology
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| Lab |
Jae-Hyuk Yu
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| Street address |
1550 Linden drive
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| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53705 |
| Country |
USA |
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| Platforms (1) |
| GPL32392 |
Illumina NovaSeq 6000 (Aspergillus nidulans) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE217820 |
Unraveling the Gene Regulatory Networks of the Global Regulators VeA and LaeA in Aspergillus nidulans |
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| Relations |
| BioProject |
PRJNA900597 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE217815_Allsamples_readcount.txt.gz |
186.9 Kb |
(ftp)(http) |
TXT |
| GSE217815_RAW.tar |
410.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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