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| Status |
Public on Jul 01, 2011 |
| Title |
Responses of fully proliferating Arabidopsis leaves to short-term osmotic stress |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Drought is an important environmental factor affecting plant growth and biomass production. Despite this importance little is known on the molecular mechanisms regulating plant growth under water limiting conditions. The main goal of this work was to investigate, using a combination of growth and molecular profiling techniques, how stress arrests CELl proliferation in Arabidopsis thaliana leaves upon osmotic stress imposition.
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| Overall design |
Plants were grown on nylon meshes overlaying control 0.5MS media. At 9 DAS when third leaf is fully proliferating seedlings were transfered to either mannitol (25mM) or control plates, and proliferating leaves were micro-dissected at 1.5h, 3h, 12h and 24h after stress imposition and using RNAlater solution (25mM). Samples were from three independent biological experiments. Each sample was pooled from multiple plants and multiple plates in one experiment. RNA samples were submitted to ATH1 array hybridization.
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| Contributor(s) |
Skirycz A, Inzé D |
| Citation(s) |
21558544 |
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| Submission date |
Jun 02, 2010 |
| Last update date |
Jan 18, 2013 |
| Contact name |
aleksandra skirycz |
| E-mail(s) |
[email protected]
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| Organization name |
Ghent University
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| Department |
Plant System Biology
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| Street address |
Technologiepark 927
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| City |
Gent |
| ZIP/Postal code |
9052 |
| Country |
Belgium |
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| Platforms (1) |
| GPL8455 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array - custom CDF (tinesath1cdf) |
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| Samples (24)
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| GSM549601 |
proliferating leaf, control, 1.5h, biological rep1 |
| GSM549602 |
proliferating leaf, control, 1.5h, biological rep2 |
| GSM549603 |
proliferating leaf, control, 1.5h, biological rep3 |
| GSM549604 |
proliferating leaf, mannitol, 1.5h, biological rep1 |
| GSM549605 |
proliferating leaf, mannitol, 1.5h, biological rep2 |
| GSM549606 |
proliferating leaf, mannitol, 1.5h, biological rep3 |
| GSM549607 |
proliferating leaf, control, 3h, biological rep1 |
| GSM549608 |
proliferating leaf, control, 3h, biological rep2 |
| GSM549609 |
proliferating leaf, control, 3h, biological rep3 |
| GSM549610 |
proliferating leaf, mannitol, 3h, biological rep1 |
| GSM549611 |
proliferating leaf, mannitol, 3h, biological rep2 |
| GSM549612 |
proliferating leaf, mannitol, 3h, biological rep3 |
| GSM549613 |
proliferating leaf, control, 12h, biological rep1 |
| GSM549614 |
proliferating leaf, control, 12h, biological rep2 |
| GSM549615 |
proliferating leaf, control, 12h, biological rep3 |
| GSM549616 |
proliferating leaf, mannitol, 12h, biological rep1 |
| GSM549617 |
proliferating leaf, mannitol, 12h, biological rep2 |
| GSM549618 |
proliferating leaf, mannitol, 12h, biological rep3 |
| GSM549619 |
proliferating leaf, control, 24h, biological rep1 |
| GSM549620 |
proliferating leaf, control, 24h, biological rep2 |
| GSM549621 |
proliferating leaf, control, 24h, biological rep3 |
| GSM549622 |
proliferating leaf, mannitol, 24h, biological rep1 |
| GSM549623 |
proliferating leaf, mannitol, 24h, biological rep2 |
| GSM549624 |
proliferating leaf, mannitol, 24h, biological rep3 |
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| Relations |
| BioProject |
PRJNA128907 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22107_RAW.tar |
51.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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