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| Status |
Public on May 16, 2023 |
| Title |
Bovine blastocyst like structures derived from stem cell cultures |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Understanding blastocyst formation and implantation is critical for improving farm animal reproduction but is hampered by a limited supply of embryos. We developed an efficient method to generate bovine blastocyst-like structures (termed blastoids) via the assembly of trophoblast stem cells and expanded potential stem cells. Bovine blastoids resemble blastocysts in morphology, cell composition, single-cell transcriptomes, and represent an accessible in vitro model for studying bovine embryogenesis.
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| Overall design |
Bovine trophoblast stem cells (TSCs) and bovine and extended pluripotent stem cells (EPSCs), were aggregated in tFACL+PD and collected at day 4.Single-cell RNA-Seq was performed using the 10x Genomics Chromium Chip and sequenced by Illumina NextSeq 500/550 sequencing systems.
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| Web link |
https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(23)00121-2
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| Contributor(s) |
Pinzón-Arteaga CA, Wang Y, Wei Y, Ribeiro Orsi AE |
| Citation(s) |
37146582 |
| |
| Submission date |
Dec 18, 2022 |
| Last update date |
May 19, 2023 |
| Contact name |
Carlos Pinzon |
| E-mail(s) |
[email protected], [email protected], [email protected]
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| Phone |
+1 3057132571
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| Organization name |
UT Southwestern Medical Center
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| Department |
Molecular Biology
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| Lab |
Jun Wu Lab
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| Street address |
5323 Harry Hines Boulevard, NA5.124
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| City |
Dallas |
| State/province |
Texas |
| ZIP/Postal code |
75390-9148 |
| Country |
USA |
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| Platforms (1) |
| GPL26012 |
Illumina NovaSeq 6000 (Bos taurus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA913592 |
