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| Status |
Public on Dec 27, 2022 |
| Title |
HOXA9 forms a repressive complex with nuclear matrix-associated protein SAFB to maintain acute myeloid leukemia [CUT&RUN/ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
This study identifies HOXA9 protein interactome in acute myeloid leukemia cells. Integrated genomic, transcriptomic and proteomic analyses further demonstrated that the HOXA9-SAFB-chromatin complex associates with NuRD and HP1γ to repress the expression of factors associated with differentiation and apoptosis in leukemia maintenance.
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| Overall design |
Fusion oncoprotein MLL-AF9 positive acute myeloid leukemia cell line MOLM13 and primary AML cells are used for all the genomics experiments. Genome-wide binding of 5 chromatin factors (HOXA9, SAFB, MTA2, GATAD2A. HP1g) and 3 histone H3 modifications (H3K27me3, H3K9me2, H3K9me3) were investigated using CUT&RUN approach in MOLM13 cells. All experiments performed and sequenced in replicates.
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| Contributor(s) |
Huntly BJ, Agrawal Singh S, Azazi D |
| Citation(s) |
36577137 |
| |
| Submission date |
Dec 23, 2022 |
| Last update date |
Mar 28, 2023 |
| Contact name |
George Giotopoulos |
| Organization name |
University of Cambridge
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| Department |
Wellcome-MRC Cambridge Stem Cell Institute - Department of Haematology
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| Lab |
Brian Huntly
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| Street address |
Jeffrey Cheah Biomedical Centre, Puddicombe Way
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| City |
Cambridge |
| State/province |
Cambridgeshire |
| ZIP/Postal code |
CB2 0AW |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (52)
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| This SubSeries is part of SuperSeries: |
| GSE221701 |
HOXA9 forms a repressive complex with nuclear matrix-associated protein SAFB to maintain acute myeloid leukemia |
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| Relations |
| BioProject |
PRJNA915391 |
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